PRODUCT CODE: ET1606-4

Recombinant MMP2 Monoclonal Antibody (ET1606-4)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MMP2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MMP2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MMP2 antibody. Counter stained with hematoxylin.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant MMP2 Monoclonal Antibody (ET1606-4)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SI15-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

72 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

72 kDa type IV collagenase

GENE NAME

MMP2

SYNONYMS

MMP-2

SEQUENCE SIMILARITIES

Belongs to the peptidase M10A family.

TISSUE SPECIFICITY

Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.; The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.

SUBCELLULAR LOCATION

[Isoform 1]: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Note=Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.; [Isoform 2]: Cytoplasm. Mitochondrion.

FUNCTION

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.; PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.; [Isoform 2]: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.