Applications
-
WB
-
ICC
-
IF
-
IHC-P
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant MMP11 Monoclonal Antibody (ET1611-33)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
293T cell lysates, SW480, MCF-7, A549, human spleen tissue, human breast carcinoma tissue, mouse spleen tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN74-08
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
55 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000
-
ICC/IF
-
1:500-1:1,000
-
IHC-P
-
1:100-1:500
TARGET
UNIPROT #
PROTEIN NAME
MMP11
SYNONYMS
Matrix Metalloproteinase 11 antibody; Matrix metalloproteinase-11 antibody; MMP-11 antibody; Mmp11 antibody; MMP11_HUMAN antibody; SL 3 antibody; SL-3 antibody; SL3 antibody; ST3 antibody; STMY3 antibody; Stromelysin 3 antibody; Stromelysin III antibody; Stromelysin-3 antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase M10A family.
TISSUE SPECIFICITY
Specifically expressed in stromal cells of breast carcinomas.
POST-TRANSLATIONAL MODIFICATION
The precursor is cleaved by a furin endopeptidase.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including Collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-3, MMP-10 and MMP-11 (also called stromelysin-1, -2 and -3) activate procollagenase. MMP-3 activation of procollagenase can occur via two pathways. Direct activation by MMP-3 is slow and activation by MMP-3 in conjunction with tissue or plasma proteinases is rapid. MMP-10 is expressed in small intestine, and at lower levels in lung and heart. MMP-11 is specifically expressed in stromal cells of breast carcinomas and contributes to epithelial cell malignancies.