PRODUCT CODE: ET1601-25

Recombinant MLKL Monoclonal Antibody (ET1601-25)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of MLKL on different cell lysates using anti-MLKL antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: HT29 <br />
Lane 2: HUVEC <br />
Lane 3: Hela
  • Western blot analysis of MLKL on different cell lysates using anti-MLKL antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: HT29 <br />
Lane 2: HUVEC <br />
Lane 3: Hela
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MLKL antibody. Counter stained with hematoxylin.
Western blot analysis of MLKL on different cell lysates using anti-MLKL antibody at 1/1,000 dilution.
Positive control:
Lane 1: HT29
Lane 2: HUVEC
Lane 3: Hela

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant MLKL Monoclonal Antibody (ET1601-25)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HUVEC, HT29, Hela, HepG2, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SA40-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

54 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Mixed lineage kinase domain-like protein

GENE NAME

MLKL

SYNONYMS

hMLKL

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.

SUBCELLULAR LOCATION

Cytoplasm. Cell membrane. Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction.

FUNCTION

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function.