PRODUCT CODE: ET1702-01

Recombinant Mitofusin 1 Monoclonal Antibody (ET1702-01)

  • Recombinant

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

Western blot analysis of Mitofusin 1 on K562 cells lysates using anti-Mitofusin 1 antibody at 1/1,000 dilution.
  • Western blot analysis of Mitofusin 1 on K562 cells lysates using anti-Mitofusin 1 antibody at 1/1,000 dilution.
  • Flow cytometric analysis of 293 cells with Mitofusin 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of Mitofusin 1 on K562 cells lysates using anti-Mitofusin 1 antibody at 1/1,000 dilution.

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Mitofusin 1 Monoclonal Antibody (ET1702-01)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF0954

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

84 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Mitofusin 1

SYNONYMS

Fzo homolog antibody; Hfzo1 antibody; Hfzo2 antibody; MFN 1 antibody; Mfn1 antibody; MFN1_HUMAN antibody; Mitochondrial transmembrane GTPase Fzo 1 antibody; Mitochondrial transmembrane GTPase FZO 2 antibody; Mitochondrial transmembrane GTPase FZO1B antibody; Mitofusin 1 antibody; Mitofusin-1 antibody; Mitofusin1 antibody; MS996 antibody; Putative transmembrane GTPase antibody; Transmembrane GTPase MFN1 antibody

SEQUENCE SIMILARITIES

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.

TISSUE SPECIFICITY

Detected in kidney and heart (at protein level). Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated by non-degradative ubiquitin by PRKN. Deubiquitination by USP30 inhibits mitochondrial fusion (By similarity). Ubiquitinated by MARCHF5. When mitochondria are depolarized and dysfunctional, it is ubiquitinated by a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that contains FBXO7 and PRKN.

SUBCELLULAR LOCATION

Mitochondrion outer membrane, Cytoplasm.

FUNCTION

Mitofusin 1 (Mfn1) and mitofusin 2 (Mfn2) are homologs for the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins and are mediators of mitochondrial fusion. A GTPase domain is required for Mfn protein function but the molecular mechanisms of the GTPase-dependent reaction as well as the functional division of the two Mfn proteins are unknown. They are essential for embryonic development and may play a role in the pathobiology of obesity. Although the Mfn1 and Mfn2 genes are broadly expressed, they show different levels of expression in different tissues. Two Mfn1 transcripts are elevated in heart, while Mfn2 mRNA is abundantly expressed in heart and muscle tissue but present only at low levels in many other tissues. Mfn1 localizes to mitochondria and participates in at least two different high molecular weight protein complexes in a GTP-dependent manner. Purified recombinant Mfn1 exhibited approximately eightfold higher GTPase activity than Mfn2.