PRODUCT CODE: ET1704-07

Recombinant MINA53 Monoclonal Antibody (ET1704-07)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

Western blot analysis of MINA53 on PC-12 cells lysates using anti-MINA53 antibody at 1/500 dilution.
  • Western blot analysis of MINA53 on PC-12 cells lysates using anti-MINA53 antibody at 1/500 dilution.
Western blot analysis of MINA53 on PC-12 cells lysates using anti-MINA53 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant MINA53 Monoclonal Antibody (ET1704-07)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA11-63

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

53 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

MINA53

SYNONYMS

60S ribosomal protein L27a histidine hydroxylase antibody; Bifunctional lysine specific demethylase and histidyl hydroxylase MINA antibody; FLJ14393 antibody; Histone lysine demethylase MINA antibody; MDIG antibody; MINA antibody; MINA_HUMAN antibody; Mineral dust-induced gene protein antibody; Myc induced nuclear antigen 53 kDa antibody; MYC induced nuclear antigen antibody; MYC-induced nuclear antigen antibody; NO52 antibody; Nucleolar protein 52 antibody; Ribosomal oxygenase MINA antibody; ROX antibody

SEQUENCE SIMILARITIES

Belongs to the ROX family. MINA53 subfamily.

TISSUE SPECIFICITY

Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. Also catalyzes the hydroxylation of 60S ribosomal protein L27a on 'His-39'. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.