PRODUCT CODE: ET1703-89

Recombinant MIF Monoclonal Antibody (ET1703-89)

  • Recombinant

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of MIF on different cells lysates using anti-MIF antibody at 1/500 dilution.<br />
Positive control: <br />
 Line 1: Mouse brain <br />
 Line 2: Jurkat <br />
 Line 3: Hela
  • Western blot analysis of MIF on different cells lysates using anti-MIF antibody at 1/500 dilution.<br />
Positive control: <br />
 Line 1: Mouse brain <br />
 Line 2: Jurkat <br />
 Line 3: Hela
  • Flow cytometric analysis of THP-1 cells with MIF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of MIF on different cells lysates using anti-MIF antibody at 1/500 dilution.
Positive control:
Line 1: Mouse brain
Line 2: Jurkat
Line 3: Hela

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant MIF Monoclonal Antibody (ET1703-89)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, Hela, THP-1, mouse brain.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-64

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

12 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IP

  • 1:10-1:50 FC

TARGET

UNIPROT #

PROTEIN NAME

Macrophage migration inhibitory factor

GENE NAME

MIF

SYNONYMS

MIF, GIF

SEQUENCE SIMILARITIES

Belongs to the MIF family.

SUBCELLULAR LOCATION

Secreted. Cytoplasm. Note=Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.

FUNCTION

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.