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PRODUCT CODE: ET1704-13

Recombinant MHC Class II Monoclonal Antibody (ET1704-13)

  • Recombinant

Applications

  • WB

  • IP

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MHC Class II antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-13, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MHC Class II antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-13, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MHC Class II antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-13, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MHC Class II antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-13, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications

  • WB

  • IP

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant MHC Class II Monoclonal Antibody (ET1704-13)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human tonsil tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA10-94

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

29 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IP

  • 1:10-1:50 ICC/IF

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

P04440

PROTEIN NAME

MHC Class II

SYNONYMS

D6S221E antibody; DMA antibody; DMB antibody; DP beta 1 antibody; DP beta 1 chain antibody; DP(W4) beta chain antibody; DPB 1 antibody; DPB1 antibody; DPB1_HUMAN antibody; DRB antibody; H2Ea antibody; HLA class II histocompatibility antigen antibody; HLA class II histocompatibility antigen DM beta chain antibody; HLA class II histocompatibility antigen, DP beta 1 chain antibody; HLA class II histocompatibility antigen, DP(W4) beta chain antibody; HLA DMB antibody; HLA DP1A antibody; HLA DPB1 antibody; HLA SB alpha chain antibody; HLA-A antibody; HLA-A histocompatibility type antibody; HLA-DP antibody; HLA-DP histocompatibility type, beta-1 subunit antibody; HLA-DP1B antibody; HLA-DPB antibody; HLA-DPB1 antibody; HLADM antibody; HLADP1B antibody; HLASB antibody; HLASB histocompatibility type antibody; Human MHC class II HLA SB alpha antibody; LA class II histocompatibility antigen DP alpha 1 chain antibody; Major histocompatibility complex class II antibody; Major histocompatibility complex class II DP alpha 1 antibody; Major histocompatibility complex class II DP beta 1 antibody; Major histocompatibility complex, class I, A antibody; MHC class II antigen DMB antibody; MHC class II antigen DPB1 antibody; MHC class II DP3 alpha antibody; MHC class II DPA1 antibody; MHC class II HLA-DP-beta-1 antibody; MHC DPB1 antibody; MHC HLA DPB1 antibody; PLT1 antibody; Primed lymphocyte test 1 antibody; RING6 antibody; RING7 antibody

SEQUENCE SIMILARITIES

Belongs to the MHC class II family.

SUBCELLULAR LOCATION

Cell membrane. Endoplasmic reticulum membrane.

FUNCTION

Major histocompatibility complex (MHC) molecules, also designated human leukocyte antigen (HLA) molecules, are cell-surface receptors that bind foreign peptides and present them to T lymphocytes. MHC class I molecules consist of two polypeptide chains, an α or heavy chain and β-2-Microglobulin, a non-covalently associated protein. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically eight to ten residues in length and are stabilized in a peptide binding groove. MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an α and β chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumu-lating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.