PRODUCT CODE: ET1701-55

Recombinant MGMT Monoclonal Antibody (ET1701-55)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of MGMT on different lysates using anti-MGMT antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: MCF-7 <br />
Lane 2: Hela
  • Western blot analysis of MGMT on different lysates using anti-MGMT antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: MCF-7 <br />
Lane 2: Hela
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGMT antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MGMT antibody. Counter stained with hematoxylin.
Western blot analysis of MGMT on different lysates using anti-MGMT antibody at 1/1,000 dilution.
Positive control:
Lane 1: MCF-7
Lane 2: Hela

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant MGMT Monoclonal Antibody (ET1701-55)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7, Hela, human tonsil tissue, human lung cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ089-6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

22 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Methylated-DNA--protein-cysteine methyltransferase

GENE NAME

MGMT

SYNONYMS

MGMT

SEQUENCE SIMILARITIES

Belongs to the MGMT family.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.