PRODUCT CODE: ET1610-47

Recombinant MelanA Monoclonal Antibody (ET1610-47)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of MelanA on different lysates using anti-MelanA antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Melanoma <br />
Lane 2: B16-F1
  • Western blot analysis of MelanA on different lysates using anti-MelanA antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Melanoma <br />
Lane 2: B16-F1
  • Flow cytometric analysis of B16-F1 cells with MelanA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the seconda antibody
Western blot analysis of MelanA on different lysates using anti-MelanA antibody at 1/1,000 dilution.
Positive control:
Lane 1: Melanoma
Lane 2: B16-F1

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant MelanA Monoclonal Antibody (ET1610-47)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

B16-F1, Melanoma.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC56-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

20 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Melanoma antigen recognized by T-cells 1

GENE NAME

MLANA

SYNONYMS

MLANA

TISSUE SPECIFICITY

Expression is restricted to melanoma and melanocyte cell lines and retina.

POST-TRANSLATIONAL MODIFICATION

Acylated.

SUBCELLULAR LOCATION

Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Note=Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.

FUNCTION

Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.