PRODUCT CODE: ET1612-98

Recombinant MEKK3 Monoclonal Antibody (ET1612-98)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of MEKK3 on different lysates using anti-MEKK3 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: 293T <br />
Lane 3: A431
  • Western blot analysis of MEKK3 on different lysates using anti-MEKK3 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: 293T <br />
Lane 3: A431
  • ICC staining MEKK3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MEKK3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MEKK3 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-MEKK3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-MEKK3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with MEKK3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of MEKK3 on different lysates using anti-MEKK3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: 293T
Lane 3: A431

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant MEKK3 Monoclonal Antibody (ET1612-98)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, 293T, MCF-7, Hela, SKOV-3, rat uterus tissue, mouse uterus tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD0839

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

71 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Mitogen-activated protein kinase kinase kinase 3

GENE NAME

MAP3K3

SYNONYMS

MEK kinase 3, MEKK 3, MAP3K3

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-166 and Ser-337 by SGK1 inhibits its activity.

FUNCTION

Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.