Lane 1: Hela
Lane 2: 293T
Lane 3: A431
Recombinant Rabbit monoclonal primary
Recombinant MEKK3 Monoclonal Antibody (ET1612-98)
293T cell lysate, A431 cell lysate, Hela, MCF-7, SKOV-3, mouse uterus tissue, rat uterus tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
M3K3_HUMAN antibody; MAP/ERK kinase kinase 3 antibody; MAP3K3 antibody; MAPK/ERK kinase kinase 3 antibody; MAPKKK3 antibody; MEK kinase 3 antibody; MEKK 3 antibody; MEKK3 antibody; Mitogen activated protein kinase kinase kinase 3 antibody; Mitogen-activated protein kinase kinase kinase 3 antibody
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Phosphorylation at Ser-166 and Ser-337 by SGK1 inhibits its activity.
MAP kinase kinase kinase (MEKK3 or MAP3K3) is a serine/threonine protein kinase that activates SAPK and ERK via phosphorylation and activation of their respective MAP kinase kinases, SEK and MEK1/2. MEKK3 also stimulates MEK5 via activation of ERK5/BMK1, which is at least partly regulated by a direct interaction between MEK5 and MEKK3 via p67phox-Bem1p (PB1) protein-protein interaction domains found in both proteins. MEKK3 modulates NF-κB activation in response to a variety of agonists including TNFα, LPS, IL-1 and LPA. Despite reports showing that phosphorylation of MEKK3 at Ser526 within the activation loop is necessary for kinase activation, at least one study suggests that dual phosphorylation at Thr516 and Ser520 is required for LPA-stimulated IKKβ/NF-κB activation. Phosphorylation at Thr294 appears to negatively regulate MEKK3 by promoting 14-3-3β binding and inhibition of the kinase activity.