PRODUCT CODE: ET1610-70

Recombinant MEKK2 Monoclonal Antibody (ET1610-70)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of MEKK2 on different lysates using anti-MEKK2 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: HepG2 <br />
Lane 2: Rat brain <br />
Lane 3: SW480
  • Western blot analysis of MEKK2 on different lysates using anti-MEKK2 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: HepG2 <br />
Lane 2: Rat brain <br />
Lane 3: SW480
  • ICC staining MEKK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MEKK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MEKK2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MEKK2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MEKK2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with MEKK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of MEKK2 on different lysates using anti-MEKK2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Rat brain
Lane 3: SW480

Applications

  • WB

  • ICC

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant MEKK2 Monoclonal Antibody (ET1610-70)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SW480, MCF-7, HepG2, mouse brain tissue, human breast carcinoma tissue, rat brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC68-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

70 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Mitogen-activated protein kinase kinase kinase 2

GENE NAME

MAP3K2

SYNONYMS

MEK kinase 2, MEKK 2, MAP3K2

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylated.; Ubiquitination by XIAP/BIRC4 does not lead to proteasomal degradation.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Note=Upon EGF stimulation, translocates into the nucleus.

FUNCTION

Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics.