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PRODUCT CODE: ET1612-35

Recombinant MEK7 Monoclonal Antibody (ET1612-35)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of MEK7 on different lysates using anti-MEK7 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: Hela <br /> Lane 2: 293T <br /> Lane 3: A431
  • Western blot analysis of MEK7 on different lysates using anti-MEK7 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: Hela <br /> Lane 2: 293T <br /> Lane 3: A431
  • ICC staining MEK7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MEK7 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MEK7 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-MEK7 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-MEK7 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-MEK7 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with MEK7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of MEK7 on different lysates using anti-MEK7 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: 293T
Lane 3: A431

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant MEK7 Monoclonal Antibody (ET1612-35)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, SHG-44, Hela, SH-SY-5Y, rat brain tissue, rat bladder tissue, mouse bladder tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD20-87

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

47 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

O14733

PROTEIN NAME

MEK7

SYNONYMS

c-Jun N-terminal kinase kinase 2 antibody; Dual specificity mitogen activated protein kinase kinase 7 antibody; Dual specificity mitogen-activated protein kinase kinase 7 antibody; JNK activating kinase 2 antibody; JNK kinase 2 antibody; JNK-activating kinase 2 antibody; JNKK 2 antibody; Jnkk-2 antibody; Jnkk2 antibody; MAP kinase kinase 7 antibody; MAP2K7 antibody; MAPK/ERK kinase 7 antibody; MAPKK 7 antibody; MAPKK-7 antibody; MAPKK7 antibody; MEK 7 antibody; Mitogen Activated Protein Kinase kinase 7 antibody; MKK 7 antibody; MKK-7 antibody; MKK7 antibody; MP2K7_HUMAN antibody; PRKMK 7 antibody; PRKMK-7 antibody; PRKMK7 antibody; SAPK kinase 4 antibody; SAPKK-4 antibody; SAPKK4 antibody; Sek 2 antibody; Sek-2 antibody; Sek2 antibody; SKK4 antibody; stress-activated protein kinase kinase 4 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

TISSUE SPECIFICITY

Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.

POST-TRANSLATIONAL MODIFICATION

Activated by phosphorylation on Ser-271 and Thr-275 by MAP kinase kinase kinases (MAP3Ks).

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.