Positive control:
Lane 1: Hela
Lane 2: 293T
Lane 3: A431
Applications
-
WB
-
ICC
-
IF
-
IHC-P
-
IP
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant MEK7 Monoclonal Antibody (ET1612-35)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
A431, SHG-44, Hela, SH-SY-5Y, rat brain tissue, rat bladder tissue, mouse bladder tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SD20-87
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
47 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
MEK7
SYNONYMS
c-Jun N-terminal kinase kinase 2 antibody; Dual specificity mitogen activated protein kinase kinase 7 antibody; Dual specificity mitogen-activated protein kinase kinase 7 antibody; JNK activating kinase 2 antibody; JNK kinase 2 antibody; JNK-activating kinase 2 antibody; JNKK 2 antibody; Jnkk-2 antibody; Jnkk2 antibody; MAP kinase kinase 7 antibody; MAP2K7 antibody; MAPK/ERK kinase 7 antibody; MAPKK 7 antibody; MAPKK-7 antibody; MAPKK7 antibody; MEK 7 antibody; Mitogen Activated Protein Kinase kinase 7 antibody; MKK 7 antibody; MKK-7 antibody; MKK7 antibody; MP2K7_HUMAN antibody; PRKMK 7 antibody; PRKMK-7 antibody; PRKMK7 antibody; SAPK kinase 4 antibody; SAPKK-4 antibody; SAPKK4 antibody; Sek 2 antibody; Sek-2 antibody; Sek2 antibody; SKK4 antibody; stress-activated protein kinase kinase 4 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
TISSUE SPECIFICITY
Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.
POST-TRANSLATIONAL MODIFICATION
Activated by phosphorylation on Ser-271 and Thr-275 by MAP kinase kinase kinases (MAP3Ks).
SUBCELLULAR LOCATION
Nucleus, Cytoplasm.
FUNCTION
A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.