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PRODUCT CODE: ET1612-35

Recombinant MEK7 Monoclonal Antibody (ET1612-35)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of MEK7 on different lysates using anti-MEK7 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: Hela <br /> Lane 2: 293T <br /> Lane 3: A431
  • Western blot analysis of MEK7 on different lysates using anti-MEK7 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: Hela <br /> Lane 2: 293T <br /> Lane 3: A431
  • ICC staining MEK7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MEK7 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MEK7 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-MEK7 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-MEK7 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-MEK7 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with MEK7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of MEK7 on different lysates using anti-MEK7 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: 293T
Lane 3: A431

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant MEK7 Monoclonal Antibody (ET1612-35)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, SHG-44, Hela, SH-SY-5Y, rat brain tissue, rat bladder tissue, mouse bladder tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD20-87

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

47 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

O14733

PROTEIN NAME

Dual specificity mitogen-activated protein kinase kinase 7

GENE NAME

MAP2K7

SYNONYMS

MAP kinase kinase 7, MAPKK 7, MEK 7, SAPK kinase 4, SAPKK-4, SAPKK4, JNK kinase 2, JNKK 2, MAP2K7

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

TISSUE SPECIFICITY

Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.

POST-TRANSLATIONAL MODIFICATION

Activated by phosphorylation on Ser-271 and Thr-275 by MAP kinase kinase kinases (MAP3Ks).

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Part of a non-canonical MAPK signaling pathway, composed of the upstream MAP3K12 kinase and downstream MAP kinases MAPK1/ERK2 and MAPK3/ERK1, that enhances the AP-1-mediated transcription of APP in response to APOE.