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PRODUCT CODE: ET1612-34

Recombinant MEK5 Monoclonal Antibody (ET1612-34)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

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Western blot analysis of MEK5 on Hela cells lysates using anti-MEK5 antibody at 1/1,000 dilution.
  • Western blot analysis of MEK5 on Hela cells lysates using anti-MEK5 antibody at 1/1,000 dilution.
  • ICC staining MEK5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MEK5 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MEK5 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of MEK5 on Hela cells lysates using anti-MEK5 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant MEK5 Monoclonal Antibody (ET1612-34)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela cell lysates, Hela, A431, SW480.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD208-6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

49 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

Q13163

PROTEIN NAME

MEK5

SYNONYMS

Dual specificity mitogen activated protein kinase kinase 5 antibody; Dual specificity mitogen-activated protein kinase kinase 5 antibody; EC 2.7.12.2 antibody; HsT17454 antibody; MAP kinase kinase 5 antibody; MAP kinase kinase MEK5b antibody; MAP2K5 antibody; MAPK/ERK kinase 5 antibody; MAPKK 5 antibody; MAPKK5 antibody; MEK 5 antibody; mitogen-activated protein kinase kinase 5 antibody; MKK5 antibody; MP2K5_HUMAN antibody; PRKMK5 antibody; Protein kinase, mitogen-activated, kinase 5 antibody; SAPKK5 antibody; SKK5 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

TISSUE SPECIFICITY

Expressed in many adult tissues. Abundant in heart and skeletal muscle.

POST-TRANSLATIONAL MODIFICATION

Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.; Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.

SUBCELLULAR LOCATION

Spindle, Cytosol, nucleus, cytoplasm.

FUNCTION

A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.