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PRODUCT CODE: ET1701-48

Recombinant MEK3+MEK6 Monoclonal Antibody (ET1701-48)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of MEK3+MEK6 on different lysates using anti-MEK3+MEK6 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: Hela <br /> Lane 2: Jurkat
  • Western blot analysis of MEK3+MEK6 on different lysates using anti-MEK3+MEK6 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: Hela <br /> Lane 2: Jurkat
  • ICC staining MEK3+MEK6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MEK3+MEK6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MEK3+MEK6 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MEK3+MEK6 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with MEK3+MEK6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of MEK3+MEK6 on different lysates using anti-MEK3+MEK6 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant MEK3+MEK6 Monoclonal Antibody (ET1701-48)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, NIH/3T3, Hela, HepG2, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ087-09

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

39/37 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P46734

PROTEIN NAME

MEK3+MEK6

SYNONYMS

Dual specificity mitogen activated protein kinase kinase 6 antibody; Dual specificity mitogen-activated protein kinase kinase 3 antibody; MAP kinase kinase 3 antibody; MAP kinase kinase 6 antibody; map2k3 antibody; MAP2K6 antibody; MAPK/ERK kinase 3 antibody; MAPK/ERK kinase 6 antibody; MAPKK 3 antibody; MAPKK 6 antibody; MAPKK3 antibody; MAPKK6 antibody; MEK 3 antibody; MEK 6 antibody; Mitogen activated protein kinase kinase 3 antibody; Mitogen activated protein kinase kinase 6 antibody; MKK3 antibody; MKK6 antibody; MP2K3_HUMAN antibody; PRKMK3 antibody; PRKMK6 antibody; Protein kinase, mitogen activated, kinase 6 (MAP kinase kinase 6) antibody; SAPK kinase 2 antibody; SAPKK 3 antibody; SAPKK-2 antibody; SAPKK2 antibody; SAPKK3 antibody; Stress activated protein kinase kinase 3 antibody; Stress-activated protein kinase kinase 2 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

TISSUE SPECIFICITY

Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity. Phosphorylated by TAOK2.; Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.