Lane 1: HepG2 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: NIH/3T3 cell lysate
Recombinant Rabbit monoclonal primary
Recombinant MEK1/2 Monoclonal Antibody (ET1602-3)
Recombinant protein within human mek1 aa 1-370.
HepG2 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, A549, NIH/3T3, human kidney tissue, mouse lung tissue, mouse kidney tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
Dual specificity mitogen-activated protein kinase kinase 2
MAP kinase kinase 1, MAPKK 1, MKK1, MEK 1, MAP kinase kinase 2, MAPKK 2, MEK 2
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1 (By similarity).; Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane localization is probably regulated by its interaction with KSR1.
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity). Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation.