Positive control:
Lane 1: HepG2 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: NIH/3T3 cell lysate
Applications
-
WB
-
ICC
-
IF
-
IHC-P
-
IP
REACTIVITY
-
Human
-
Mouse
-
Rat
-
Zebrafish
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant MEK1/2 Monoclonal Antibody (ET1602-3)
Immunogen
Recombinant protein within human mek1 aa 1-370.
Host
Rabbit
Positive Control
HepG2 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, A549, NIH/3T3, human kidney tissue, mouse lung tissue, mouse kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SR13-07
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
44 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
IP
-
assay-dependent
TARGET
UNIPROT #
PROTEIN NAME
MEK1/2
SYNONYMS
AA589381 antibody; CFC3 antibody; Dual specificity mitogen-activated protein kinase kinase 1 antibody; Dual specificity mitogen-activated protein kinase kinase 2 antibody; EC 2.7.12.2 antibody; ERK activator kinase 1 antibody; ERK activator kinase 2 antibody; FLJ26075 antibody; MAP kinase kinase 1 antibody; MAP kinase kinase 2 antibody; MAP2K1 antibody; MAP2K2 antibody; MAPK/ERK kinase 1 antibody; MAPK/ERK kinase 2 antibody; MAPKK 1 antibody; MAPKK1 antibody; MAPKK2 antibody; MEK 1 antibody; MEK1 antibody; MEKK1 antibody; Mitogen activated protein kinase kinase 1 antibody; Mitogen activated protein kinase kinase 2 antibody; Mitogen-activated protein kinase kinase 2, p45 antibody; MK2 antibody; MKK 1 antibody; MKK 2 antibody; MKK1 antibody; MKK2 antibody; MP2K1_HUMAN antibody; PRKMK 1 antibody; PRKMK 2 antibody; Prkmk1 antibody; Prkmk2 antibody; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) antibody; Protein kinase, mitogen-activated, kinase 1 antibody; Protein kinase, mitogen-activated, kinase 2 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
POST-TRANSLATIONAL MODIFICATION
MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1 (By similarity).; Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Membrane, Mitochondrion
FUNCTION
A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.