PRODUCT CODE: ET7107-04

Recombinant MEIS2 Monoclonal Antibody (ET7107-04)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of MEIS2 on different lysates using anti-MEIS2 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse testis <br />
  Lane 2: SH-SY-5Y<br />
  Lane 3: Mouse lung
  • Western blot analysis of MEIS2 on different lysates using anti-MEIS2 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse testis <br />
  Lane 2: SH-SY-5Y<br />
  Lane 3: Mouse lung
  • Flow cytometric analysis of K562 cells with MEIS2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of MEIS2 on different lysates using anti-MEIS2 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse testis
Lane 2: SH-SY-5Y
Lane 3: Mouse lung

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant MEIS2 Monoclonal Antibody (ET7107-04)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse testis tissue lysate, SH-SY-5Y, human kidney tissue lysate, mouse lung tissue lysate, K562.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB40-38

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

51 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

MEIS2

SYNONYMS

Homeobox protein Meis2 antibody; HsT18361 antibody; Meis (mouse) homolog 2 antibody; Meis homeobox 2 antibody; Meis homolog 2 antibody; Meis1 myeloid ecotropic viral integration site 1 homolog 2 (mouse) antibody; Meis1 myeloid ecotropic viral integration site 1 homolog 2 antibody; Meis1 related gene 1 antibody; Meis1 related protein 1 antibody; Meis1-related protein 1 antibody; MEIS2 antibody; MEIS2_HUMAN antibody; MGC2820 antibody; MRG1 antibody; TALE homeobox protein Meis2 antibody

SEQUENCE SIMILARITIES

Belongs to the TALE/MEIS homeobox family.

TISSUE SPECIFICITY

Expressed in various tissues. Expressed at high level in the lymphoid organs of hematopoietic tissues. Also expressed in some regions of the brain, such as the putamen.

DEVELOPMENTAL STAGE

Expressed in the proliferative zones of the fetal neocortex. Expressed at a very high level in the developing ganglionic eminence and at a more moderate level in the cortical plate.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

Profilins regulate Actin polymerization by binding to and sequestering the Actin monomer. Profilins act as a nucleotide exchange factor that charges Actin with ATP after binding the Actin monomer through a 1:1 stoichiometric relationship. Human Profilin-1 and Profilin-2 are encoded by two separate genes mapping to chromosomes 17p13.2 and 3q25.1, respectively. Both Profilin-1 and Profilin-2 are abundantly expressed in kidney. Profilin-1 is highly expressed in lung, liver, placenta and kidney while Profilin-2 is highly expressed in brain and skeletal muscle. In axonal and dendritic processes of mouse brain, Profilins co-localize with dynamin I and synapsin. Profilin may play a role in mediating cell adhesion. The overexpression of Profilin in endothelial cells results in increased adhesion to Fibronectin. In food allergy, plant Profilin is considered a pan-allergen. Case studies indicate individuals with allergies to various foods including celery, carrots, zucchini and peanuts are actually sensitive to the Profilin proteins in these foods.