PRODUCT CODE: ET7107-15

Recombinant MCM5 Monoclonal Antibody (ET7107-15)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

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+
Western blot analysis of MCM5 on different lysates using anti-MCM5 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse thymus tissue<br />
  Lane 2: SiHa
  • Western blot analysis of MCM5 on different lysates using anti-MCM5 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse thymus tissue<br />
  Lane 2: SiHa
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCM5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MCM5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MCM5 antibody. Counter stained with hematoxylin.
Western blot analysis of MCM5 on different lysates using anti-MCM5 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse thymus tissue
Lane 2: SiHa

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant MCM5 Monoclonal Antibody (ET7107-15)

Immunogen

Recombinant protein within c terminal human mcm5.

Host

Rabbit

Positive Control

Mouse thymus tissue lysate, SiHa, human tonsil tissue, human placenta tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB31-34

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

82 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

MCM5

SYNONYMS

CDC 46 antibody; CDC46 antibody; CDC46 homolog antibody; Cell division cycle 46 antibody; DNA replication licensing factor antibody; DNA replication licensing factor MCM 5 antibody; DNA replication licensing factor MCM5 antibody; MCM 5 antibody; MCM5 antibody; MCM5_HUMAN antibody; MGC5315 antibody; Minichromosome maintenance complex component 5 antibody; Minichromosome maintenance deficient (S. cerevisiae) 5 antibody; Minichromosome maintenance deficient 5 antibody; Minichromosome maintenance deficient protein 5 antibody; P1 CDC46 antibody; P1-CDC46 antibody

SEQUENCE SIMILARITIES

Belongs to the MCM family.

SUBCELLULAR LOCATION

Nucleus. Cytosol.

FUNCTION

The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation during apoptosis of the MCM complex, which is composed of, at least, MCM2–6.