PRODUCT CODE: ET7107-15

Recombinant MCM5 Monoclonal Antibody (ET7107-15)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of MCM5 on different lysates using anti-MCM5 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse thymus tissue<br />
 Lane 2: SiHa<br />
  • Western blot analysis of MCM5 on different lysates using anti-MCM5 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse thymus tissue<br />
 Lane 2: SiHa<br />
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCM5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MCM5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MCM5 antibody. Counter stained with hematoxylin.
Western blot analysis of MCM5 on different lysates using anti-MCM5 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse thymus tissue
Lane 2: SiHa

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant MCM5 Monoclonal Antibody (ET7107-15)

Immunogen

Recombinant protein within c terminal human mcm5.

Host

Rabbit

Positive Control

Mouse thymus tissue lysate, SiHa, human tonsil tissue, human placenta tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB31-34

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

82 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

DNA replication licensing factor MCM5

GENE NAME

MCM5

SYNONYMS

MCM5, CDC46

SEQUENCE SIMILARITIES

Belongs to the MCM family.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm, cytosol.

FUNCTION

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Interacts with MCMBP.