Lane 1: Raji cell lysate
Lane 2: A431 tissue lysate
Recombinant Rabbit monoclonal primary
Recombinant MCL1 Monoclonal Antibody (ET1606-14)
Raji cell lysate, a431 tissue lysate, mouse heart tissue lysates, Hela, HepG2, BT-20, human breast tissue, human kidney tissue, human pancreas tissue, Jurkat.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
Bcl 2 related protein EAT/mcl1 antibody; Bcl-2-like protein 3 antibody; Bcl-2-related protein EAT/mcl1 antibody; BCL2 related antibody; Bcl2-L-3 antibody; BCL2L3 antibody; EAT antibody; Induced myeloid leukemia cell differentiation protein Mcl 1 antibody; Induced myeloid leukemia cell differentiation protein Mcl-1 antibody; MCL 1 antibody; MCL1 antibody; MCL1-ES antibody; mcl1/EAT antibody; MCL1_HUMAN antibody; MCL1L antibody; MCL1S antibody; MGC104264 antibody; MGC1839 antibody; Myeloid Cell Leukemia 1 antibody; Myeloid cell leukemia ES antibody; Myeloid cell leukemia sequence 1 antibody; Myeloid cell leukemia sequence 1 BCL2 related antibody; Myeloid cell leukemia sequence 1 isoform 1 antibody; OTTHUMP00000032794 antibody; OTTHUMP00000032795 antibody; TM antibody
Belongs to the Bcl-2 family.
Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.; Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.; Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.; Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159.
Membrane, Cytoplasm, Mitochondrion, Nucleus.
B-cell CLL/lymphoma 2 (Bcl-2) blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Myeloid cell leukemia 1 (Mcl-1) shares sequence homology with Bcl-2 and further resembles Bcl-2 in that its expression promotes cell viability. p53 and Mcl-1 demonstrate opposing effects on mitochondrial apoptosis by mediating Bcl-2 antagonist killer (Bak) activity. Mcl-1 is an important and specific regulator that is necessary for the homeostasis of early hematopoietic progenitors. Glycogen synthase kinase 3 (GSK3) controls Mcl-1 stability, which has an effect on the regulation of apoptosis by growth factors, PI 3-kinase and AKT. Mice with a deficiency of the Mcl-1 protein show a significant reduction in B and T lymphocytes similar to the effects observed in IL-7- or IL-7R-deficient mice.