PRODUCT CODE: ET7106-95

Recombinant Matrin 3 Monoclonal Antibody (ET7106-95)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Matrin 3 on different lysates using anti-Matrin 3 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Daudi<br />
 Lane 2: Mouse brain tissue<br />
  • Western blot analysis of Matrin 3 on different lysates using anti-Matrin 3 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Daudi<br />
 Lane 2: Mouse brain tissue<br />
  • ICC staining Matrin 3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Matrin 3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Matrin 3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Matrin 3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Matrin 3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Matrin 3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Matrin 3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Matrin 3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of LOVO cells with Matrin 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Matrin 3 on different lysates using anti-Matrin 3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Daudi
Lane 2: Mouse brain tissue

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Matrin 3 Monoclonal Antibody (ET7106-95)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Daudi, mouse brain tissue lysate, Hela, LOVO, SH-SY-5Y, rat brain tissue, human tonsil tissue, human lung cancer tissue, human liver tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU93-43

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

95 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Matrin-3

GENE NAME

MATR3

SYNONYMS

MATR3

SUBCELLULAR LOCATION

Nucleus matrix.

FUNCTION

May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the SFPQ-NONO heteromer may play a role in nuclear retention of defective RNAs. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. May bind to specific miRNA hairpins.