PRODUCT CODE: ET7107-19

Recombinant Mast Cell Chymase Monoclonal Antibody (ET7107-19)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of Mast Cell Chymase on rat spleen and mouse spleen tissue lysates using anti-Mast Cell Chymase antibody at 1/500 dilution.
  • Western blot analysis of Mast Cell Chymase on rat spleen and mouse spleen tissue lysates using anti-Mast Cell Chymase antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti- Mast Cell Chymase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- Mast Cell Chymase antibody. Counter stained with hematoxylin.
Western blot analysis of Mast Cell Chymase on rat spleen and mouse spleen tissue lysates using anti-Mast Cell Chymase antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Mast Cell Chymase Monoclonal Antibody (ET7107-19)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat spleen and mouse spleen tissue lysates, Human tonsil tissue, human skin tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB74-32

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

27kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Chymase

GENE NAME

CMA1

SYNONYMS

CMA1, CYH, CYM

SEQUENCE SIMILARITIES

Belongs to the peptidase S1 family. Granzyme subfamily.

TISSUE SPECIFICITY

Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.

SUBCELLULAR LOCATION

Secreted. Cytoplasmic granule. Note=Mast cell granules.

FUNCTION

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.