Applications
-
WB
-
IP
REACTIVITY
-
Human
-
Mouse
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant MAPKAP Kinase 2 Monoclonal Antibody (ET7106-83)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
SK-Br-3.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JU30-31
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
46 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
IP
-
1:10-1:50
TARGET
UNIPROT #
PROTEIN NAME
MAPKAP Kinase 2
SYNONYMS
MAP kinase activated protein Kinase 2 antibody; MAP kinase-activated protein kinase 2 antibody; MAPK activated protein kinase 2 antibody; MAPK-activated protein kinase 2 antibody; MAPK2_HUMAN antibody; MAPKAP K2 antibody; MAPKAP kinase 2 antibody; MAPKAPK 2 antibody; MAPKAPK-2 antibody; MAPKAPK2 antibody; Mitogen activated protein kinase activated protein kinase 2 antibody; MK 2 antibody; MK2 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
TISSUE SPECIFICITY
Expressed in all tissues examined.
POST-TRANSLATIONAL MODIFICATION
Sumoylation inhibits the protein kinase activity.; Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-222, Ser-272 and Thr-334.
SUBCELLULAR LOCATION
Cytoplasm. Nucleus.
FUNCTION
The MAPKAP kinases (for MAP kinase activated protein kinases) are a group of MAP kinase substrates which are themselves kinases. In response to activation, the MAP kinases phosphorylate downstream components on a consensus Pro-X-Ser/Thr-Pro motif. Several kinases that contain this motif have been identifed and serve as substrates for the ERK and p38 MAP kinases. These include the serine/threonine kinases Rsk-1 (also designated MAPKAP kinase-1), Rsk-2 and Rsk-3, which are phosphorylated by ERK1 and ERK2. Similarly, p38 phosphorylates and activates the serine/threonine kinases MAPKAP kinase-2 and MAPKAP kinase-3 (also designated 3pK). The serine/threonine kinases Mnk1 and Mnk2 are substrates for both ERK and p38 MAP kinases.