PRODUCT CODE: ET7106-83

Recombinant MAPKAP Kinase 2 Monoclonal Antibody (ET7106-83)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

Western blot analysis of MAPKAP Kinase 2 on SK-Br-3 cell lysate using anti-MAPKAP Kinase 2 antibody at 1/500 dilution.
  • Western blot analysis of MAPKAP Kinase 2 on SK-Br-3 cell lysate using anti-MAPKAP Kinase 2 antibody at 1/500 dilution.
Western blot analysis of MAPKAP Kinase 2 on SK-Br-3 cell lysate using anti-MAPKAP Kinase 2 antibody at 1/500 dilution.

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant MAPKAP Kinase 2 Monoclonal Antibody (ET7106-83)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SK-Br-3.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU30-31

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

46 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

MAPKAP Kinase 2

SYNONYMS

MAP kinase activated protein Kinase 2 antibody; MAP kinase-activated protein kinase 2 antibody; MAPK activated protein kinase 2 antibody; MAPK-activated protein kinase 2 antibody; MAPK2_HUMAN antibody; MAPKAP K2 antibody; MAPKAP kinase 2 antibody; MAPKAPK 2 antibody; MAPKAPK-2 antibody; MAPKAPK2 antibody; Mitogen activated protein kinase activated protein kinase 2 antibody; MK 2 antibody; MK2 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

TISSUE SPECIFICITY

Expressed in all tissues examined.

POST-TRANSLATIONAL MODIFICATION

Sumoylation inhibits the protein kinase activity.; Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-222, Ser-272 and Thr-334.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

The MAPKAP kinases (for MAP kinase activated protein kinases) are a group of MAP kinase substrates which are themselves kinases. In response to activation, the MAP kinases phosphorylate downstream components on a consensus Pro-X-Ser/Thr-Pro motif. Several kinases that contain this motif have been identifed and serve as substrates for the ERK and p38 MAP kinases. These include the serine/threonine kinases Rsk-1 (also designated MAPKAP kinase-1), Rsk-2 and Rsk-3, which are phosphorylated by ERK1 and ERK2. Similarly, p38 phosphorylates and activates the serine/threonine kinases MAPKAP kinase-2 and MAPKAP kinase-3 (also designated 3pK). The serine/threonine kinases Mnk1 and Mnk2 are substrates for both ERK and p38 MAP kinases.