Positive control:
Lane 1: SHG-44 cell lysate
Lane 2: mouse brain tissue lysate
Lane 3: mouse liver tissue lysate
Lane 4: mouse skeletal muscle tissue lysate
Applications
-
WB
-
ICC
-
IF
-
IHC-P
-
IP
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant MAP1LC3A Monoclonal Antibody (ET1609-26)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
SHG-44 cell lysate, mouse brain tissue lysate, mouse liver tissue lysate, mouse skeletal muscle tissue lysate, Hela, PC-12, HUVEC, human liver tissue, mouse liver tissue, mouse brain tissue, SH-SY5Y.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
ST47-03
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
14 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
MAP1LC3A
SYNONYMS
ATG8E antibody; Autophagy-related protein LC3 A antibody; Autophagy-related ubiquitin-like modifier LC3 A antibody; LC3 antibody; LC3A antibody; MAP1 light chain 3 like protein 1 antibody; MAP1 light chain 3-like protein 1 antibody; MAP1A/1B light chain 3 A antibody; MAP1A/MAP1B LC3 A antibody; MAP1A/MAP1B light chain 3 A antibody; MAP1ALC3 antibody; MAP1BLC3 antibody; Map1lc3a antibody; Microtubule associated proteins 1A/1B light chain 3 antibody; Microtubule-associated protein 1 light chain 3 alpha antibody; Microtubule-associated proteins 1A and 1B, light chain 3 antibody; Microtubule-associated proteins 1A/1B light chain 3A antibody; MLP3A_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the ATG8 family.
TISSUE SPECIFICITY
Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
POST-TRANSLATIONAL MODIFICATION
The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.; The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.; Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.
SUBCELLULAR LOCATION
Cytoplasm, Endomembrane system, Cytoplasmic vesicle.
FUNCTION
Microtubules, the primary component of the cytoskeletal network, interact with proteins called microtubule-associated proteins (MAPs). The microtubule-associated proteins can be divided into two groups, structural and dynamic. The structural microtubule-associated proteins, MAP-1A, MAP-1B, MAP-2A, MAP-2B and MAP-2C, stimulate tubulin assembly, enhance micro-tubule stability and influence the spatial distribution of microtubules within cells. Both MAP-1 and, to a greater extent, MAP-2 have been implicated as agents of microtubule depolymerization by suppressing the dynamic instability of the microtubules. The suppression of microtubule dynamic instability by the MAP proteins is thought to be associated with phosphorylation of the MAPs.