PRODUCT CODE: ET1702-04

Recombinant Mannose Receptor (CD206) Monoclonal Antibody (ET1702-04)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

Western blot analysis of Mannose Receptor(CD206) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-04, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: Human lung tissue lysate<br />
 Lane 2: HepG2 cell lysate<br />
 Lane 3: 293 cell lysate
  • Western blot analysis of Mannose Receptor(CD206) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-04, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: Human lung tissue lysate<br />
 Lane 2: HepG2 cell lysate<br />
 Lane 3: 293 cell lysate
  • Western blot analysis of Mannose Receptor(CD206) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-04, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: Mouse lung tissue lysate<br />
 Lane 2: D3 cell lysate
  • ICC staining of Mannose Receptor(CD206) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-04, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of Mannose Receptor(CD206) in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-04, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of Mannose Receptor(CD206) in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-04, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of Mannose Receptor(CD206) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-04, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human lung tissue lysate
Lane 2: HepG2 cell lysate
Lane 3: 293 cell lysate

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Mannose Receptor (CD206) Monoclonal Antibody (ET1702-04)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human lung tissue lysate, HepG2 cell lysate, 293 cell lysate, mouse lung tissue lysate, D3 cell lysate, Hela, MCF-7, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF0953

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

166/140 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

Mannose Receptor(CD206)

SYNONYMS

bA541I19.1 antibody; C type lectin domain family 13 member D antibody; C-type lectin domain family 13 member D antibody; CD 206 antibody; CD206 antibody; CD206 antigen antibody; CLEC13D antibody; CLEC13DL antibody; Macrophage mannose receptor 1 antibody; Macrophage mannose receptor 1 like protein 1 antibody; Macrophage mannose receptor antibody; Mannose receptor C type 1 antibody; Mannose receptor C type 1 like 1 antibody; MMR antibody; MRC 1 antibody; MRC1 antibody; MRC1_HUMAN antibody; MRC1L1 antibody; OTTHUMP00000045206 antibody

SUBCELLULAR LOCATION

Endosome membrane, Cell membrane.

FUNCTION

CD206, also known as macrophage mannose receptor type C (MMR or MRC1), is a type I membrane receptor protein. It is an phagocytic and endocytic receptor that can recognize carbohydrate ligands in target molecules. The extracellular portion of the protein includes eight C-type carbohydrate recognition domains (CRD) which are clustered together to achieve higher affinity binding to saccharides. CD206 is found on macrophages and on endothelial cells of the liver and is the only known example of a C-type lectin that contains multiple C-type CRDs. CD206 mediates the endocytosis of glycoproteins by macrophages and binds high-mannose structures on the surface of potentially pathogenic viruses, fungi and bacteria enabling them to be neutralized by phagocytic engulfment. During inflammation, CD206 is crucial for rapid clearance of several mannose-bearing serum glycoproteins but does not regulate the initiation of inflammation. CD206 is primarily expressed in mature tissue macrophages and immature dendritic cells, as well as hepatic and lymphatic endothelial cells, retinal pigmental epithelium (RPE) and mesangial cells.