PRODUCT CODE: ET1706-38

Recombinant Mad2L2 Monoclonal Antibody (ET1706-38)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Mad2L2 on SiHa cell using anti-Mad2L2 antibody at 1/500 dilution.
  • Western blot analysis of Mad2L2 on SiHa cell using anti-Mad2L2 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Mad2L2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Mad2L2 on SiHa cell using anti-Mad2L2 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Mad2L2 Monoclonal Antibody (ET1706-38)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SiHa, rat lung tissue, mouse colon tissue, human tonsil tissue, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU99-23

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

24 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Mitotic spindle assembly checkpoint protein MAD2B

GENE NAME

MAD2L2

SYNONYMS

MAD2-like protein 2, hREV7, MAD2L2, MAD2B, REV7

TISSUE SPECIFICITY

Ubiquitously expressed.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Chromosome. Note=Recruited to sites of chromosomal double-stranded breaks during G1 and S phase of the cell cycle.

FUNCTION

Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. Component of the shieldin complex, which plays an important role in repair of DNA double-stranded breaks (DSBs). During G1 and S phase of the cell cycle, the complex functions downstream of TP53BP1 to promote non-homologous end joining (NHEJ) and suppress DNA end resection. Mediates various NHEJ-dependent processes including immunoglobulin class-switch recombination, and fusion of unprotected telomeres. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.