PRODUCT CODE: ET7106-69

Recombinant Mad2L1 Monoclonal Antibody (ET7106-69)

  • Recombinant

Applications

  • WB

  • ICC

REACTIVITY

  • Human

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+
Western blot analysis of Mad2L1 on different cell lysates using anti-Mad2L1 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: K562 <br />
  Lane 2: 293T<br />
  Lane 3: SH-SY5Y
  • Western blot analysis of Mad2L1 on different cell lysates using anti-Mad2L1 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: K562 <br />
  Lane 2: 293T<br />
  Lane 3: SH-SY5Y
  • ICC staining Mad2L1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of Mad2L1 on different cell lysates using anti-Mad2L1 antibody at 1/500 dilution.
Positive control:
Lane 1: K562
Lane 2: 293T
Lane 3: SH-SY5Y

Applications

  • WB

  • ICC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Mad2L1 Monoclonal Antibody (ET7106-69)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562, 293T, SH-SY5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU46-13

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

23.5 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

Mad2L1

SYNONYMS

Caught by MAD2 protein antibody; CMT2 antibody; dJ261G23.1 antibody; KIAA0110 antibody; MAD2L1 binding protein antibody; MAD2L1 binding protein isoform 2 antibody; MAD2L1-binding protein antibody; Mad2l1bp antibody; MD2BP_HUMAN antibody; MGC11282 antibody; OTTHUMP00000016496 antibody; RP1 261G23.6 antibody

SEQUENCE SIMILARITIES

Belongs to the MAD2 family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20. Phosphorylated by NEK2.

SUBCELLULAR LOCATION

Nucleus. Cytoskeleton.

FUNCTION

Cell cycle progression is subject to arrest at the mitotic spindle assembly checkpoint in response to incorrect spindle fiber assembly. MAD2 (for mitotic arrest-deficient) is a component of the mitotic spindle checkpoint. Cells with mutated MAD2 do not undergo mitotic arrest in response to incorrect spindle fiber assembly, which results in missegregation and eventual cell death. A breast carcinoma cell line with reduced MAD2 expression, T47D, was shown to complete mitosis in the presence of nocodazole, an inhibitor of mitotic spindle assembly. MAD2 is localized to unattached kinetochores during prometaphase and disassociates upon spindle fiber attachment, indicating that MAD2 regulates kinetochore binding to the spindle fibers. Human MAD2 has also been shown to associate with insulin receptor (IR), but not IGFIR, implicating MAD2 as a mediator for IR-specific signaling. MAD2B, a MAD2 homolog, is required for the execution of the mitotic checkpoint monitoring the kinetochore-spindle attachment process and if the process is not complete, MAD2B delays the onset of anaphase.