PRODUCT CODE: ET1706-15

Recombinant macroH2A.1 Monoclonal Antibody (ET1706-15)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of macroH2A.1 on different lysates using anti-macroH2A.1 antibody at 1/500 dilution.<br />
 Positive control:<br />
  Lane 1: Hela<br />
  Lane 2: Mouse placenta tissue<br />
  Lane 3: MCF-7
  • Western blot analysis of macroH2A.1 on different lysates using anti-macroH2A.1 antibody at 1/500 dilution.<br />
 Positive control:<br />
  Lane 1: Hela<br />
  Lane 2: Mouse placenta tissue<br />
  Lane 3: MCF-7
  • ICC staining macroH2A.1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining macroH2A.1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining macroH2A.1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.
Western blot analysis of macroH2A.1 on different lysates using anti-macroH2A.1 antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: Mouse placenta tissue
Lane 3: MCF-7

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant macroH2A.1 Monoclonal Antibody (ET1706-15)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, mouse placenta tissue lysate, MCF-7, HepG2, PC-3M, human lung cancer tissue, human thyroid grand, human placenta tissue, mouse fallopian tube tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU22-83

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

40 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

macroH2A.1

SYNONYMS

Core histone macro h2a.1 antibody; Core histone macro-H2A.1 antibody; H2A histone family member Y antibody; H2A.y antibody; H2A/y antibody; H2AF12M antibody; H2AFJ antibody; H2afy antibody; H2AY_HUMAN antibody; Histone H2A.Y antibody; Histone macroH2A1 antibody; Histone macroH2A1.1 antibody; Histone macroH2A1.2 antibody; Macroh2a1 antibody; MACROH2A1.1 antibody; MacroH2A1.2 antibody; Medulloblastoma antigen MU MB 50.205 antibody; Medulloblastoma antigen MU-MB-50.205 antibody; mH2a antibody; mH2A1 antibody

TISSUE SPECIFICITY

Widely expressed.

POST-TRANSLATIONAL MODIFICATION

Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.