PRODUCT CODE: ET1704-51

Recombinant LXR alpha Monoclonal Antibody (ET1704-51)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of LXR alpha on mouse colon tissue lysate using anti-LXR alpha antibody at 1/1,000 dilution.
  • Western blot analysis of LXR alpha on mouse colon tissue lysate using anti-LXR alpha antibody at 1/1,000 dilution.
  • ICC staining LXR alpha in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-LXR alpha antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-LXR alpha antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-LXR alpha antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with LXR alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of LXR alpha on mouse colon tissue lysate using anti-LXR alpha antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant LXR alpha Monoclonal Antibody (ET1704-51)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human liver tissue lysates, HepG2, human placenta tissue, mouse testis tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA20-38

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

LXR alpha

SYNONYMS

Liver X receptor alpha antibody; LXR a antibody; LXRA antibody; NR1H3 antibody; NR1H3_HUMAN antibody; Nuclear receptor subfamily 1 group H member 3 antibody; Oxysterols receptor LXR alpha antibody; Oxysterols receptor LXR-alpha antibody; RLD 1 antibody; RLD1 antibody

SEQUENCE SIMILARITIES

Belongs to the nuclear hormone receptor family. NR1 subfamily.

TISSUE SPECIFICITY

Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated leading to its degradation by the proteasome.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.