PRODUCT CODE: ET1704-51

Recombinant LXR alpha Monoclonal Antibody (ET1704-51)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of LXR alpha on mouse colon tissue lysate using anti-LXR alpha antibody at 1/1,000 dilution.
  • Western blot analysis of LXR alpha on mouse colon tissue lysate using anti-LXR alpha antibody at 1/1,000 dilution.
  • ICC staining LXR alpha in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-LXR alpha antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-LXR alpha antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-LXR alpha antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with LXR alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of LXR alpha on mouse colon tissue lysate using anti-LXR alpha antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant LXR alpha Monoclonal Antibody (ET1704-51)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse colon tissue lysate, Human spleen tissue, human placenta tissue, mouse testes tissue, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA20-38

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Oxysterols receptor LXR-alpha

GENE NAME

NR1H3

SYNONYMS

NR1H3, LXRA

SEQUENCE SIMILARITIES

Belongs to the nuclear hormone receptor family. NR1 subfamily.

TISSUE SPECIFICITY

Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated leading to its degradation by the proteasome.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES (By similarity). LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides (By similarity). Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity).