PRODUCT CODE: ET1706-31

Recombinant LOX Monoclonal Antibody (ET1706-31)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

Western blot analysis of LOX on different lysates using anti-LOX antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Jurkat <br />
 Lane 2: Mouse brain<br />
 Lane 3: Human fetal brain<br />
  • Western blot analysis of LOX on different lysates using anti-LOX antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Jurkat <br />
 Lane 2: Mouse brain<br />
 Lane 3: Human fetal brain<br />
  • ICC staining LOX in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining LOX in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining LOX in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-LOX antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LOX antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-LOX antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-LOX antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with LOX antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
  • Western blot analysis of LOX on Zebrafish tissue lysates using anti-LOX antibody at 1/1,000 dilution.
Western blot analysis of LOX on different lysates using anti-LOX antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: Mouse brain
Lane 3: Human fetal brain

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant LOX Monoclonal Antibody (ET1706-31)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, mouse brain tissue lysate, human fetal brain tissue, Zebrafish tissue lysates, Hela, HUVEC, SH-SY5Y, rat skeletal tissue, human kidney tissue, human placenta tissue, mouse heart tissue, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU30-23

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Protein-lysine 6-oxidase

GENE NAME

LOX

SYNONYMS

LOX

SEQUENCE SIMILARITIES

Belongs to the lysyl oxidase family.

TISSUE SPECIFICITY

Heart, placenta, skeletal muscle, kidney, lung and pancreas.

POST-TRANSLATIONAL MODIFICATION

The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.; Proteolytically cleaved by BMP1 which removes the propeptide. Also proteolytically cleaved by ADAMTS2 and ADAMTS14, but not by ADAMTS3, at an additional cleavage site downstream of the BMP1 cleavage site. The propeptide plays a role in directing the deposition of this enzyme to elastic fibers, via interaction with tropoelastin (By similarity). Cleavage by BMP1 to remove the propeptide does not increase enzymatic activity but increases binding to collagen. Cleavage by ADAMTS2 produces a form with reduced collagen-binding activity.; Sulfated at Tyr-187 and also at either Tyr-183 or Tyr-184 which enhances binding to collagen.

SUBCELLULAR LOCATION

Secreted. Secreted, extracellular space.

FUNCTION

Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture (By similarity).