PRODUCT CODE: ET1704-48

Recombinant Lipoprotein lipase Monoclonal Antibody (ET1704-48)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of Lipoprotein lipase on human placenta tissue lysate using anti-Lipoprotein lipase antibody at 1/1,000 dilution.
  • Western blot analysis of Lipoprotein lipase on human placenta tissue lysate using anti-Lipoprotein lipase antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human ileum tissue using anti-Lipoprotein lipase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Lipoprotein lipase antibody. Counter stained with hematoxylin.
Western blot analysis of Lipoprotein lipase on human placenta tissue lysate using anti-Lipoprotein lipase antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Lipoprotein lipase Monoclonal Antibody (ET1704-48)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human placenta tissue lysate, human ileum tissue, human pancreas tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA22-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

53 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Lipoprotein lipase

SYNONYMS

EC 3.1.1 antibody; EC 3.1.1.34 antibody; HDLCQ11 antibody; LIPD antibody; LIPL_HUMAN antibody; Lipoprotein lipase antibody; LPL antibody; LPL protein antibody; MGC137861 antibody

SEQUENCE SIMILARITIES

Belongs to the AB hydrolase superfamily. Lipase family.

TISSUE SPECIFICITY

Detected in blood plasma. Detected in milk (at protein level).

POST-TRANSLATIONAL MODIFICATION

Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.

SUBCELLULAR LOCATION

Cell membrane. Secreted.

FUNCTION

The Lipase gene family belongs to one of the most robust genetic superfamilies found in living organisms, which includes esterases and thioesterases. Lipase gene products are related by tertiary structure rather than primary amino acid sequence. Members of the AB hydrolase subfamily include hepatic lipase (HL), endothelial lipase (EL), lipoprotein lipase (LPL) and pancreatic lipase (PL). HL balances the composition and transport of lipoproteins in human plasma. Synthesized in endothelial cells, EL hydrolyzes high density lipoproteins. LPL, a homodimer attached to the membrane by a GPI-anchor, mediates the hydrolysis of triglycerides of very low density lipoproteins and circulating chylomicrons. Defects in LPL may cause chylomicronemia syndrome or a form of lipoprotein lipase deficiency characterized by hypertriglyceridemia.