PRODUCT CODE: ET1703-39

Recombinant Lipocalin-2 Monoclonal Antibody (ET1703-39)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of Lipocalin-2 on A431 cells lysates using anti-Lipocalin-2 antibody at 1/500 dilution.
  • Western blot analysis of Lipocalin-2 on A431 cells lysates using anti-Lipocalin-2 antibody at 1/500 dilution.
  • ICC staining Lipocalin-2 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Lipocalin-2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Lipocalin-2 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Lipocalin-2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Lipocalin-2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Lipocalin-2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Lipocalin-2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Lipocalin-2 antibody. Counter stained with hematoxylin.
Western blot analysis of Lipocalin-2 on A431 cells lysates using anti-Lipocalin-2 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Lipocalin-2 Monoclonal Antibody (ET1703-39)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, 293T, Hela, SW480, human stomach cancer tissue, human spleen tissue, human uterus tissue, human lung tissue, human pancreas tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-67

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

23 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Neutrophil gelatinase-associated lipocalin

GENE NAME

LCN2

SYNONYMS

NGAL, LCN2

SEQUENCE SIMILARITIES

Belongs to the calycin superfamily. Lipocalin family.

TISSUE SPECIFICITY

Detected in neutrophils (at protein level). Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.

SUBCELLULAR LOCATION

Secreted. Cytoplasmic granule lumen. Cytoplasmic vesicle lumen. Note=Upon binding to the SLC22A17 (24p3R) receptor, it is internalized (By similarity). Releases the bound iron in the acidic lumen of cytoplasmic vesicles.

FUNCTION

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis (By similarity). Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin. Can also bind siderophores from M.tuberculosis.