PRODUCT CODE: ET1703-39

Recombinant Lipocalin-2 Monoclonal Antibody (ET1703-39)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of Lipocalin-2 on A431 cells lysates using anti-Lipocalin-2 antibody at 1/500 dilution.
  • Western blot analysis of Lipocalin-2 on A431 cells lysates using anti-Lipocalin-2 antibody at 1/500 dilution.
  • ICC staining Lipocalin-2 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Lipocalin-2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Lipocalin-2 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Lipocalin-2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Lipocalin-2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Lipocalin-2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Lipocalin-2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Lipocalin-2 antibody. Counter stained with hematoxylin.
Western blot analysis of Lipocalin-2 on A431 cells lysates using anti-Lipocalin-2 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Lipocalin-2 Monoclonal Antibody (ET1703-39)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, 293T, Hela, SW480, human stomach cancer tissue, human spleen tissue, human uterus tissue, human lung tissue, human pancreas tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-67

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

23 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Lipocalin-2

SYNONYMS

24p3 antibody; 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody; 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody; Alpha 2 microglobulin related protein antibody; HGNC:6526 antibody; HNL antibody; Lcn 2 antibody; Lcn2 antibody; Lipocalin-2 antibody; Migration stimulating factor inhibitor antibody; MSFI antibody; Neutrophil gelatinase associated lipocalin antibody; Neutrophil gelatinase associated lipocalin precursor antibody; Neutrophil gelatinase-associated lipocalin antibody; NGAL antibody; NGAL_HUMAN antibody; Oncogene 24p3 antibody; Oncogenic lipocalin 24p3 antibody; p25 antibody; Siderocalin antibody; siderocalin LCN2 antibody; SV40 induced 24P3 protein antibody; Uterocalin antibody

SEQUENCE SIMILARITIES

Belongs to the calycin superfamily. Lipocalin family.

TISSUE SPECIFICITY

Detected in neutrophils (at protein level). Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

In addition to the monomeric mammalian progelatinase, two additional forms of progelatinase have been identified. The shorter of these additional forms is a covalently linked, disulfide-bridged protein that heterodimerizes with a short protein; an α2-Microglobulin-related protein known as neutrophil gelatinase-associated lipocalin (NGAL), which is moderately expressed in breast and lung tissues. NGAL belongs to the lipocalin family and has a high degree of similarity with rat α2-Microglobulin-related protein and mouse protein 24p3. NGAL is able to bind a derivative of the bacterial chemotactic peptide, suggesting that it has important immuno-modulatory functions. NGAL has been described as an inflammatory protein; it is released into the circulation as a result of the inflammatory activation of leukocytes initiated by the extra-corporeal circulation. In addition, NGAL synthesis is induced in epithelial cells in inflammatory and neoplastic colorectal diseases. In conclusion, NGAL may serve as a scavenger of bacterial products to function in the anti-inflammatory process.