PRODUCT CODE: ET1701-94

Recombinant LAMP1 Monoclonal Antibody (ET1701-94)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of LAMP1 on different lysates using anti-LAMP1 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: Jurkat
  • Western blot analysis of LAMP1 on different lysates using anti-LAMP1 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: Jurkat
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LAMP1 antibody. Counter stained with hematoxylin.
Western blot analysis of LAMP1 on different lysates using anti-LAMP1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant LAMP1 Monoclonal Antibody (ET1701-94)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, Hela, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ0940

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

120 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

LAMP1

SYNONYMS

CD107 antigen like family member A antibody; CD107 antigen-like family member A antibody; CD107a antibody; CD107a antigen antibody; LAMP 1 antibody; LAMP-1 antibody; LAMP1 antibody; LAMP1_HUMAN antibody; LAMPA antibody; LGP120 antibody; lgpA antibody; Lysosomal membrane glycoprotein 120KD antibody; Lysosomal Associated Membrane Protein 1 antibody; Lysosome associated membrane glycoprotein 1 antibody; Lysosome-associated membrane glycoprotein 1 antibody; Lysosome-associated membrane protein 1 antibody; OTTHUMP00000040663 antibody

SEQUENCE SIMILARITIES

Belongs to the LAMP family.

POST-TRANSLATIONAL MODIFICATION

O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. The glycosylation of N-76 is essential for Lassa virus entry into cells.

SUBCELLULAR LOCATION

Cell membrane, Endosome membrane, Lysosome membrane, Late endosome.

FUNCTION

Lysosome-associated membrane proteins (LAMP) are glycosylated type I membrane proteins that play a role in the biogenesis of the pigment melanin. LAMP-1 (also designated CD107a) and LAMP-2 (also designated CD107b) are involved in a variety of functions, including cellular adhesion, and are thought to participate in the process of tumor invasion and metastasis. Newly synthesized LAMP-1 and LAMP-2 proteins are sorted at the trans-Golgi network and are transported intracellularly via a pathway that is distinct from the Clathrin-coated vesicles used for the mannose-6 phosphate receptor. LAMP-1 is expressed on the surface of Thrombin-activated but not resting platelets, and it is thought to be involved in the adhesive, prothrombic properties of these cells. Both LAMP-1 and LAMP-2 are involved in maintaining lysosome acidity and protecting the lysosomal membranes from autodigestion, and their expression is increased in patients with lysosomal storage disorders.

CITATIONS

  • Zhu, Xudong et al.

    Cellular Evidence and Source of Exosomes in the Biliary System of the Chinese Soft-Shelled Turtle, Pelodiscus sinensis. | Frontiers in Physiology [2019]