PRODUCT CODE: ET1701-99

Recombinant Laminnin 5 alpha 3 Monoclonal Antibody (ET1701-99)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

Western blot analysis of Laminin 5 alpha 3 on A431 cells lysates using anti-Laminnin 5 alpha 3 antibody at 1/1,000 dilution.
  • Western blot analysis of Laminin 5 alpha 3 on A431 cells lysates using anti-Laminnin 5 alpha 3 antibody at 1/1,000 dilution.
  • ICC staining Laminin 5 alpha 3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Laminin 5 alpha 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of Laminin 5 alpha 3 on A431 cells lysates using anti-Laminnin 5 alpha 3 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Laminnin 5 alpha 3 Monoclonal Antibody (ET1701-99)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, Hela, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ0957

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

367 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

Laminin subunit alpha-3

GENE NAME

LAMA3

SYNONYMS

E170, LAMA3

TISSUE SPECIFICITY

Skin; respiratory, urinary, and digestive epithelia and in other specialized tissues with prominent secretory or protective functions. Epithelial basement membrane, and epithelial cell tongue that migrates into a wound bed. A differential and focal expression of the subunit alpha-3 is observed in the CNS.

SUBCELLULAR LOCATION

Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component.

FUNCTION

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.; Laminin-5 is thought to be involved in (1) cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes, (2) signal transduction via tyrosine phosphorylation of pp125-FAK and p80, (3) differentiation of keratinocytes.