PRODUCT CODE: ET1701-99

Recombinant Laminnin 5 alpha 3 Monoclonal Antibody (ET1701-99)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

Western blot analysis of Laminin 5 alpha 3 on A431 cells lysates using anti-Laminnin 5 alpha 3 antibody at 1/1,000 dilution.
  • Western blot analysis of Laminin 5 alpha 3 on A431 cells lysates using anti-Laminnin 5 alpha 3 antibody at 1/1,000 dilution.
  • ICC staining Laminin 5 alpha 3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Laminin 5 alpha 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of Laminin 5 alpha 3 on A431 cells lysates using anti-Laminnin 5 alpha 3 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Laminnin 5 alpha 3 Monoclonal Antibody (ET1701-99)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, Hela, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ0957

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

367 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

Laminnin 5 alpha 3

SYNONYMS

E170 antibody; Epiligrin 170 kDa subunit antibody; Epiligrin subunit alpha antibody; Kalinin subunit alpha antibody; LAMA3 antibody; LAMA3A antibody; Laminin 5 alpha3 antibody; Laminin A3 antibody; laminin alpha 3 antibody; laminin alpha3 antibody; Laminin subunit alpha-3 antibody; Laminin-5 subunit alpha antibody; Laminin-6 subunit alpha antibody; Laminin-7 subunit alpha antibody; laminin5 alpha 3 antibody; Laminin5 alpha3 antibody; LAMNA antibody; LOCS antibody; Nicein subunit alpha antibody

TISSUE SPECIFICITY

Skin; respiratory, urinary, and digestive epithelia and in other specialized tissues with prominent secretory or protective functions. Epithelial basement membrane, and epithelial cell tongue that migrates into a wound bed. A differential and focal expression of the subunit alpha-3 is observed in the CNS.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. Through interactions with integrins, dystroglycan and other receptors, laminins contribute to cell differentiation, cell shape and migration, and maintenance of tissue phenotypes and survival. Laminin alpha 3/Laminin-5, also known as epiligrin, includes alpha 3, beta 3, and gamma 2 subunits. It is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa and other epithelial glands and contributes to initiation and maintenance of epithelial cell anchorage to the underlying connective tissue. Within aa 21?1713 of the alpha 3 subunit, human and mouse share 77% amino acid sequence identity.