PRODUCT CODE: ET1703-14

Recombinant Laminin beta 1 Monoclonal Antibody (ET1703-14)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Laminin beta 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Laminin beta 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Laminin beta 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Laminin beta 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Laminin beta 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Laminin beta 1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A431 cells with Laminin beta 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Laminin beta 1 antibody. Counter stained with hematoxylin.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Laminin beta 1 Monoclonal Antibody (ET1703-14)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, human kidney tissue, mouse colon tissue, mouse prostate tissue, mouse kidney tissue, mouse small intestine tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM099-03

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

198 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • FC

  • 1:50-1:100

  • IHC-P

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

Laminin beta 1

SYNONYMS

CLM antibody; Cutis laxa with marfanoid phenotype antibody; LAM B1 antibody; LAMB 1 antibody; LAMB1 antibody; LAMB1_HUMAN antibody; Laminin B1 antibody; Laminin B1 chain antibody; Laminin beta 1 chain antibody; Laminin beta 1 chain precursor antibody; Laminin beta1 antibody; Laminin subunit beta 1 antibody; Laminin subunit beta-1 antibody; Laminin-1 subunit beta antibody; Laminin-10 subunit beta antibody; Laminin-12 subunit beta antibody; Laminin-2 subunit beta antibody; Laminin-6 subunit beta antibody; Laminin-8 subunit beta antibody; LIS5 antibody; MGC142015 antibody

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Laminins are essential and abundant structural non-collagenous glyco- proteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of laminins with stabilizing type IV collagen networks, nidogen, and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve, and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression, and tissue architecture. Each laminin is a heterotrimer of a, b, and g chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan, and cognate laminin receptors. The human Laminin b-1 gene maps to chromosome 7q22 and is ubiquitously expressed in tissues that produce basement membranes.