Positive control:
Lane 1: Hela cell lysate
Lane 2: A549 cell lysate
Lane 3: MCF-7 cell lysate
Applications
-
WB
-
ICC
-
IF
-
IHC-P
-
IP
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
-
Zebrafish
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Lactate Dehydrogenase Monoclonal Antibody (ET1608-57)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Hela cell lysate, A549 cell lysate, MCF-7 cell lysate, hybrid fish (crucian-carp) brain tissue lysates, A549, A431, human liver tissue, human liver carcinoma tissue, human breast carcinoma tissue, mouse liver tissue, mouse testis tissue, mouse skeletal muscle tissue, Hela.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SU39-06
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
37 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:200-1:500
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Lactate Dehydrogenase
SYNONYMS
Cell proliferation-inducing gene 19 protein antibody; GSD11 antibody; L lactate dehydrogenase B chain antibody; L-lactate dehydrogenase A chain antibody; Lactate dehydrogenase A antibody; Lactate dehydrogenase B antibody; Lactate dehydrogenase H chain antibody; Lactate dehydrogenase M antibody; LDH A antibody; LDH B antibody; LDH H antibody; LDH heart subunit antibody; LDH M antibody; LDH muscle subunit antibody; LDH-A antibody; LDH-M antibody; LDH1 antibody; ldha antibody; LDHA_HUMAN antibody; LDHBD antibody; LDHM antibody; MS1111 antibody; PIG19 antibody; Proliferation inducing gene 19 antibody; Renal carcinoma antigen NY REN 46 antibody; Renal carcinoma antigen NY-REN-59 antibody; TRG 5 antibody; TRG5 antibody
SEQUENCE SIMILARITIES
Belongs to the LDH/MDH superfamily. LDH family.
POST-TRANSLATIONAL MODIFICATION
ISGylated.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
The lactate dehydrogenase family (LDH) catalyzes the final step of anaerobic glycolysis, the conversion of L-lactate and NAD to pyruvate and NADH. The LDH family consists of three members, LDH-A, LDH-B and LDH-C, all of which form tetramers consisting four subunits. However, each family member displays a specific tissue distribution pattern with LDH-A and LDH-B predominant in several tissues, specifically LDH-A in muscle and LDH-B in heart, while LDH-C expression is confined to the testis and sperm. LDHs function as powerful markers for germ cell tumors. The genes encoding human LDH-A and LDH-C map to chromosome 11, while the human LDH-B gene maps to chromosome 12. Deficiency in the LDH-A gene is linked to exertional myoglobinuria.