PRODUCT CODE: ET1705-61

Recombinant KPNA2 Monoclonal Antibody (ET1705-61)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of KPNA2 on different cell lysates using anti-KPNA2 antibody at 1/1,000 dilution.<br />
Postive control:<br />
Lane 1: Hela<br />
Lane 2: 293T<br />
Lane 3: HepG2<br />
Lane 4: NIH-3T3<br />
Lane 5: PC-12
  • Western blot analysis of KPNA2 on different cell lysates using anti-KPNA2 antibody at 1/1,000 dilution.<br />
Postive control:<br />
Lane 1: Hela<br />
Lane 2: 293T<br />
Lane 3: HepG2<br />
Lane 4: NIH-3T3<br />
Lane 5: PC-12
  • Flow cytometric analysis of 293T cells with KPNA2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of KPNA2 on different cell lysates using anti-KPNA2 antibody at 1/1,000 dilution.
Postive control:
Lane 1: Hela
Lane 2: 293T
Lane 3: HepG2
Lane 4: NIH-3T3
Lane 5: PC-12

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant KPNA2 Monoclonal Antibody (ET1705-61)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2, Hela, 293T, NIH-3T3, PC-12.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM90-33

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Importin subunit alpha-1

GENE NAME

KPNA2

SYNONYMS

KPNA2, RCH1, SRP1

SEQUENCE SIMILARITIES

Belongs to the importin alpha family.

TISSUE SPECIFICITY

Expressed ubiquitously.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.