PRODUCT CODE: ET1705-59

Recombinant KMT5A/SETD8/Pr-SET7 Monoclonal Antibody (ET1705-59)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of KMT5A on different lysates using anti-KMT5A antibody at 1/1,000 dilution.<br />
Positive control:<br />
Lane 1: Mouse kidney<br />
Lane 2: Mouse liver<br />
Lane 3: 293T<br />
Lane 4: 293
  • Western blot analysis of KMT5A on different lysates using anti-KMT5A antibody at 1/1,000 dilution.<br />
Positive control:<br />
Lane 1: Mouse kidney<br />
Lane 2: Mouse liver<br />
Lane 3: 293T<br />
Lane 4: 293
  • Flow cytometric analysis of 293T cells with KMT5A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of KMT5A on different lysates using anti-KMT5A antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse kidney
Lane 2: Mouse liver
Lane 3: 293T
Lane 4: 293

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant KMT5A/SETD8/Pr-SET7 Monoclonal Antibody (ET1705-59)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse kidney tissue, mouse liver tissue, 293T, 293.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM73-43

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

N-lysine methyltransferase KMT5A

GENE NAME

KMT5A

SYNONYMS

PR-Set7, PR/SET07, KMT5A, PRSET7, SET07, SET8, SETD8

SEQUENCE SIMILARITIES

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. PR/SET subfamily.

DEVELOPMENTAL STAGE

Not detected during G1 phase. First detected during S through G2 phases, and peaks during mitosis (at protein level).

POST-TRANSLATIONAL MODIFICATION

Acetylated at Lys-172; does not change methyltransferase activity. Deacetylated at Lys-172 by SIRT2; does not change methyltransferase activity.; Ubiquitinated and degraded by the DCX(DTL) complex.

SUBCELLULAR LOCATION

Nucleus. Chromosome. Note=Specifically localizes to mitotic chromosomes. Colocalized with SIRT2 at mitotic foci. Associates with chromosomes during mitosis; association is increased in a H(2)O(2)-induced oxidative stress-dependent manner. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.

FUNCTION

Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration.