PRODUCT CODE: ET1705-88

Recombinant Klotho Monoclonal Antibody (ET1705-88)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Klotho on human serum lysate using anti-Klotho antibody at 1/1,000 dilution.
  • Western blot analysis of Klotho on human serum lysate using anti-Klotho antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Klotho antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Klotho antibody. Counter stained with hematoxylin.
Western blot analysis of Klotho on human serum lysate using anti-Klotho antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Klotho Monoclonal Antibody (ET1705-88)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human serum lysate, human kidney tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM93-76

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Klotho

GENE NAME

KL

SYNONYMS

KL

SEQUENCE SIMILARITIES

Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.

TISSUE SPECIFICITY

Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.

SUBCELLULAR LOCATION

[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Klotho peptide]: Secreted.

FUNCTION

May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity).; The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.