PRODUCT CODE: ET1702-71

Recombinant KLF4 Monoclonal Antibody (ET1702-71)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of KLF4 on different lysates using anti-KLF4 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: 293 <br />
Lane 2: NCCIT
  • Western blot analysis of KLF4 on different lysates using anti-KLF4 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: 293 <br />
Lane 2: NCCIT
  • ICC staining KLF4 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining KLF4 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining KLF4 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-KLF4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-KLF4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-KLF4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-KLF4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-KLF4 antibody. Counter stained with hematoxylin.
Western blot analysis of KLF4 on different lysates using anti-KLF4 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293
Lane 2: NCCIT

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant KLF4 Monoclonal Antibody (ET1702-71)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293, NCCIT, 293T, PC-3M, Hela, human liver tissue, mouse liver tissue, human gastric carcinoma tissue, mouse colon tissue, mouse stomach tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF98-08

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

55 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Krueppel-like factor 4

GENE NAME

KLF4

SYNONYMS

KLF4

SEQUENCE SIMILARITIES

Belongs to the krueppel C2H2-type zinc-finger protein family.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.