PRODUCT CODE: ET7107-43

Recombinant KDM5A Monoclonal Antibody (ET7107-43)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

Western blot analysis of KDM5A on A549 cell using anti-KDM5A antibody at 1/500 dilution.
  • Western blot analysis of KDM5A on A549 cell using anti-KDM5A antibody at 1/500 dilution.
  • ICC staining KDM5A in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining KDM5A in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of SH-SY-5Y cells with KDM5A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of KDM5A on A549 cell using anti-KDM5A antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant KDM5A Monoclonal Antibody (ET7107-43)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A549, SiHa, SH-SY-5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB36-60

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

192 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:400

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

KDM5A

SYNONYMS

Histone demethylase JARID1A antibody; JARID1A antibody; Jumonji/ARID domain containing protein 1A antibody; Jumonji/ARID domain-containing protein 1A antibody; Kdm5a antibody; KDM5A_HUMAN antibody; Lysine-specific demethylase 5A antibody; RBBP-2 antibody; RBBP2 antibody; RBP2 antibody; Retinoblastoma binding protein 2 antibody; Retinoblastoma-binding protein 2 antibody

SEQUENCE SIMILARITIES

Belongs to the JARID1 histone demethylase family.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Regulates specific gene transcription through DNA-binding on 5'-CCGCCC-3' motif. May stimulate transcription mediated by nuclear receptors. Involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12. Plays a role in the regulation of the circadian rhythm and in maintaining the normal periodicity of the circadian clock. In a histone demethylase-independent manner, acts as a coactivator of the CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER1/2 and other clock-controlled genes and increases histone acetylation at PER1/2 promoters by inhibiting the activity of HDAC1. Seems to act as a transcriptional corepressor for some genes such as MT1F and to favor the proliferation of cancer cells.