PRODUCT CODE: ET1610-90

Recombinant Junctional Adhesion Molecule 1 Monoclonal Antibody (ET1610-90)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of JAM-1 on different lysates using anti-JAM-1 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: Human liver
  • Western blot analysis of JAM-1 on different lysates using anti-JAM-1 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: Human liver
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-JAM-1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-JAM-1 antibody. Counter stained with hematoxylin.
Western blot analysis of JAM-1 on different lysates using anti-JAM-1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Human liver

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Junctional Adhesion Molecule 1 Monoclonal Antibody (ET1610-90)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, HepG2, human liver tissue, mouse liver tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC60-07

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

33 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Junctional adhesion molecule A

GENE NAME

F11R

SYNONYMS

JAM-A, JAM-1, PAM-1, F11R

SEQUENCE SIMILARITIES

Belongs to the immunoglobulin superfamily.

TISSUE SPECIFICITY

Expressed in endothelium, epithelium and leukocytes (at protein level).

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.

SUBCELLULAR LOCATION

Cell junction, tight junction. Cell membrane; Single-pass type I membrane protein. Note=Localized at tight junctions of both epithelial and endothelial cells.

FUNCTION

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (By similarity). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration. Involved in platelet activation.; (Microbial infection) Acts as a receptor for Mammalian reovirus sigma-1.; (Microbial infection) Acts as a receptor for Human Rotavirus strain Wa.