PRODUCT CODE: ET1705-84

Recombinant JAK1 Monoclonal Antibody (ET1705-84)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of JAK1 on Hela cell using anti-JAK1 antibody at 1/1,000 dilution.<br />
Positive control:<br />
Lane 1: PC-12<br />
Lane 2: Jurkat<br />
Lane 3: NIH-3T3
  • Western blot analysis of JAK1 on Hela cell using anti-JAK1 antibody at 1/1,000 dilution.<br />
Positive control:<br />
Lane 1: PC-12<br />
Lane 2: Jurkat<br />
Lane 3: NIH-3T3
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-JAK1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-JAK1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SW480 cells with JAK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of JAK1 on Hela cell using anti-JAK1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: PC-12
Lane 2: Jurkat
Lane 3: NIH-3T3

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant JAK1 Monoclonal Antibody (ET1705-84)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12, Jurkat, NIH-3T3, mouse colon tissue, mouse kidney tissue, SW480.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM75-03

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

133 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50 FC

TARGET

UNIPROT #

PROTEIN NAME

Tyrosine-protein kinase JAK1

GENE NAME

JAK1

SYNONYMS

JAK-1, JAK1A, JAK1B

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

TISSUE SPECIFICITY

Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylated. Phosphorylated on tyrosine residues in response to interferon gamma signaling. Dephosphorylation of Tyr-1034 and Tyr-1035 by PTPN2 negatively regulates cytokine-mediated signaling.; Ubiquitinated by RNF125; leading to its degradation by the proteasome.

SUBCELLULAR LOCATION

Endomembrane system; Peripheral membrane protein. Note=Wholly intracellular, possibly membrane associated.

FUNCTION

Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.