PRODUCT CODE: ET1610-33

Recombinant Islet 1 Monoclonal Antibody (ET1610-33)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of Islet 1 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Islet 1 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • ICC staining of Islet 1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-33, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of Islet 1 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Islet 1 Monoclonal Antibody (ET1610-33)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7 cell lysates, A549.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC05-64

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

39 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Islet 1

SYNONYMS

Insulin gene enhancer protein ISL 1 antibody; Insulin gene enhancer protein ISL-1 antibody; Insulin related protein antibody; ISL 1 antibody; ISL LIM homeobox 1 antibody; ISL1 antibody; ISL1 transcription factor LIM homeodomain antibody; ISL1 transcription factor, LIM/homeodomain (islet 1) antibody; ISL1 transcription factor, LIM/homeodomain antibody; ISL1_HUMAN antibody; Islet-1 antibody; Islet1 antibody

TISSUE SPECIFICITY

Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Islet-1 (ISL1 transcription factor, LIM/homeodomain) and Islet-2 (ISL2 transcription factor, LIM/homeodomain) contain amino-terminal LIM domains and a carboxy-terminal homeodomain and both influence developmental events. Islet-1 influences embryogenesis of the pancreatic islets of Langerhans and neural tube motor neuron differentiation. In developing mouse teeth, Islet-1 mediates patterning of dentition as an activator of Bmp4 expression in incisor (distal) areas of the stomatodeal epithelium. Islet-1 expression defines cardiac progenitor cell populations and is required for normal cardiac development and asymmetry. Islet-2 activity in newly generated motor neurons permits the diversification of visceral and somatic motor neuron subtypes in the developing spinal cord. Murine Islet-2 specifies retinal ganglion cell (RGC) laterality by repressing an ipsilateral pathfinding program unique to the ventral-temporal crescent (VTC) of RGCs in a Zic2- and EphB1-dependent manner.