Applications
-
WB
-
ICC
-
IF
-
IP
REACTIVITY
-
Human
-
Mouse
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Islet 1 Monoclonal Antibody (ET1610-33)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
MCF-7 cell lysates, A549.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC05-64
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
39 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000
-
ICC/IF
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
Islet 1
SYNONYMS
Insulin gene enhancer protein ISL 1 antibody; Insulin gene enhancer protein ISL-1 antibody; Insulin related protein antibody; ISL 1 antibody; ISL LIM homeobox 1 antibody; ISL1 antibody; ISL1 transcription factor LIM homeodomain antibody; ISL1 transcription factor, LIM/homeodomain (islet 1) antibody; ISL1 transcription factor, LIM/homeodomain antibody; ISL1_HUMAN antibody; Islet-1 antibody; Islet1 antibody
TISSUE SPECIFICITY
Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Islet-1 (ISL1 transcription factor, LIM/homeodomain) and Islet-2 (ISL2 transcription factor, LIM/homeodomain) contain amino-terminal LIM domains and a carboxy-terminal homeodomain and both influence developmental events. Islet-1 influences embryogenesis of the pancreatic islets of Langerhans and neural tube motor neuron differentiation. In developing mouse teeth, Islet-1 mediates patterning of dentition as an activator of Bmp4 expression in incisor (distal) areas of the stomatodeal epithelium. Islet-1 expression defines cardiac progenitor cell populations and is required for normal cardiac development and asymmetry. Islet-2 activity in newly generated motor neurons permits the diversification of visceral and somatic motor neuron subtypes in the developing spinal cord. Murine Islet-2 specifies retinal ganglion cell (RGC) laterality by repressing an ipsilateral pathfinding program unique to the ventral-temporal crescent (VTC) of RGCs in a Zic2- and EphB1-dependent manner.