PRODUCT CODE: ET1612-36

Recombinant IRF6 Monoclonal Antibody (ET1612-36)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of IRF6 on K562 cells lysates using anti-IRF6 antibody at 1/1,000 dilution.
  • Western blot analysis of IRF6 on K562 cells lysates using anti-IRF6 antibody at 1/1,000 dilution.
  • ICC staining IRF6 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining IRF6 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining IRF6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-IRF6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IRF6 antibody. Counter stained with hematoxylin.
Western blot analysis of IRF6 on K562 cells lysates using anti-IRF6 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant IRF6 Monoclonal Antibody (ET1612-36)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562, MCF-7, 293, SW480, human kidney tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD202-6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

60 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Interferon regulatory factor 6

GENE NAME

IRF6

SYNONYMS

IRF-6, IRF6

SEQUENCE SIMILARITIES

Belongs to the IRF family.

TISSUE SPECIFICITY

Expressed in normal mammary epithelial cells. Expression is reduced or absent in breast carcinomas.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated. Phosphorylation status depends on the cell cycle and is a signal for ubiquitination and proteasome-mediated degradation.

SUBCELLULAR LOCATION

Nucleus. Note=Translocates to nucleus in response to an activating signal.

FUNCTION

Probable DNA-binding transcriptional activator. Key determinant of the keratinocyte proliferation-differentiation switch involved in appropriate epidermal development (By similarity). Plays a role in regulating mammary epithelial cell proliferation (By similarity). May regulate WDR65 transcription (By similarity).