PRODUCT CODE: ET1612-15

Recombinant IRF5 Monoclonal Antibody (ET1612-15)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of IRF5 on THP-1 cells lysates using anti-IRF5 antibody at 1/1,000 dilution.
  • Western blot analysis of IRF5 on THP-1 cells lysates using anti-IRF5 antibody at 1/1,000 dilution.
  • ICC staining IRF5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining IRF5 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IRF5 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with IRF5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of IRF5 on THP-1 cells lysates using anti-IRF5 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant IRF5 Monoclonal Antibody (ET1612-15)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

THP-1 cell lysates, HepG2, RH-35, human spleen tissue, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD203-07

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

56 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

IRF5

SYNONYMS

Interferon regulatory factor 5 antibody; Interferon regulatory factor 5 bone marrow variant antibody; IRF 5 antibody; IRF-5 antibody; Irf5 antibody; IRF5_HUMAN antibody; SLEB10 antibody

SEQUENCE SIMILARITIES

Belongs to the IRF family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation of serine and threonine residues in a C-terminal autoinhibitory region, stimulates dimerization, transport into the nucleus, assembly with the coactivator CBP/p300 and initiation of transcription.; 'Lys-63'-linked polyubiquitination by TRAF6 is required for activation.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

Interferon regulatory factor 5 (IRF-5), belongs to the IRF family of DNA-binding factors, which includes IRF-1, IRF-2, IRF-3, IRF-4, IRF-6, IRF-7, ISGF-3γ p48 and IFN consensus sequence-binding protein (ICSBP). The IRF family regulate both interferon and interferon-inducible genes. IRF-5, like IRF-3 and IRF-7, is a direct transducer of virus-mediated signaling and plays a role in the expression of multiple cytokines/chemokines. Although IRF-5 is a direct target of p53, its cell cycle regulatory and proapoptotic effects are p53 independent.