PRODUCT CODE: ET1612-15

Recombinant IRF5 Monoclonal Antibody (ET1612-15)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of IRF5 on THP-1 cells lysates using anti-IRF5 antibody at 1/1,000 dilution.
  • Western blot analysis of IRF5 on THP-1 cells lysates using anti-IRF5 antibody at 1/1,000 dilution.
  • ICC staining IRF5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining IRF5 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IRF5 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with IRF5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of IRF5 on THP-1 cells lysates using anti-IRF5 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant IRF5 Monoclonal Antibody (ET1612-15)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

THP-1, HepG2, RH-35, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD203-07

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

56 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Interferon regulatory factor 5

GENE NAME

IRF5

SYNONYMS

IRF-5

SEQUENCE SIMILARITIES

Belongs to the IRF family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation of serine and threonine residues in a C-terminal autoinhibitory region, stimulates dimerization, transport into the nucleus, assembly with the coactivator CBP/p300 and initiation of transcription.; 'Lys-63'-linked polyubiquitination by TRAF6 is required for activation.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm.

FUNCTION

Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling.