Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant IRF5 Monoclonal Antibody (ET1611-94)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
THP-1, human lung cancer tissue, human breast carcinoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN201-05
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
56 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
FC
-
1:50-1:100
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
IRF5
SYNONYMS
Interferon regulatory factor 5 antibody; Interferon regulatory factor 5 bone marrow variant antibody; IRF 5 antibody; IRF-5 antibody; Irf5 antibody; IRF5_HUMAN antibody; SLEB10 antibody
SEQUENCE SIMILARITIES
Belongs to the IRF family.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation of serine and threonine residues in a C-terminal autoinhibitory region, stimulates dimerization, transport into the nucleus, assembly with the coactivator CBP/p300 and initiation of transcription.; 'Lys-63'-linked polyubiquitination by TRAF6 is required for activation.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Interferon regulatory factor 5 (IRF-5), belongs to the IRF family of DNA-binding factors, which includes IRF-1, IRF-2, IRF-3, IRF-4, IRF-6, IRF-7, ISGF-3γ p48 and IFN consensus sequence-binding protein (ICSBP). The IRF family regulate both interferon and interferon-inducible genes. IRF-5, like IRF-3 and IRF-7, is a direct transducer of virus-mediated signaling and plays a role in the expression of multiple cytokines/chemokines. Although IRF-5 is a direct target of p53, its cell cycle regulatory and proapoptotic effects are p53 independent.