PRODUCT CODE: ET1704-27

Recombinant IP10 Monoclonal Antibody (ET1704-27)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

ICC staining of IP10 in N2A cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-27, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of IP10 in N2A cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-27, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of IP10 in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-27, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of IP10 in SH-SY5Y cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-27, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-IP10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC staining of IP10 in N2A cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-27, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant IP10 Monoclonal Antibody (ET1704-27)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

N2A, HepG2, SH-SY5Y, human skin tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA10-82

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

10 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

IP10

SYNONYMS

Interferon gamma induced factor MOB1, mouse, homolog of antibody; Interferon gamma induced protein 10 antibody; 10 kDa interferon gamma induced protein antibody; 10 kDa interferon gamma-induced protein antibody; C X C motif chemokine 10 antibody; C7 antibody; Chemokine (C X C motif) ligand 10 antibody; Chemokine CXC motif ligand 10 antibody; Crg 2 antibody; CRG2 antibody; CXCL10 antibody; CXCL10(1-73) antibody; CXL10_HUMAN antibody; Gamma IP10 antibody; Gamma-IP10 antibody; gIP 10 antibody; GIP10 antibody; IFI10 antibody; INP 10 antibody; INP10 antibody; Interferon activated gene 10 antibody; Interferon activated gene 10 antibody; Interferon gamma induced cell line antibody; Interferon inducible cytokine IP 10 antibody; Interferon inducible cytokine IP10 antibody; IP 10 antibody; IP-10 antibody; Mob 1 antibody; MOB1 antibody; Protein 10 from interferon (gamma) induced cell line antibody; SCYB10 antibody; Small inducible cytokine B10 antibody; Small inducible cytokine B10 precursor antibody; Small inducible cytokine subfamily B (Cys X Cys) member 10 antibody; Small inducible cytokine subfamily B CXC member 10 antibody; Small inducible cytokine subfamily B, member 10 antibody; Small-inducible cytokine B10 antibody

SEQUENCE SIMILARITIES

Belongs to the intercrine alpha (chemokine CxC) family.

TISSUE SPECIFICITY

Mainly secreted by monocytes, endothelial cells as well as fibroblasts. Expressed by epithelial cells in thymus. Microglial cells produce CXCL10 in response to viral stimulation.

POST-TRANSLATIONAL MODIFICATION

Several proteases can mediate post-secretion cleavages. DPP4 cleaves CXCL10 on its N-terminal 2 amino acids leading to an antagonist form of CXCL10. This dominant negative form is capable of binding CXCR3 but does not induce signaling. MMP9 cleaves 9 amino acids instead.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Chemokines are members of a superfamily of inducible, secreted, pro-inflammatory cytokines. Members of the chemokine family exhibit 20% to 50% homology in their predicted amino acid sequences and are divided into four subfamilies: C-C, C-X-C, C and C-X3-C. In the C-X-C or α subfamily, the first two of four cysteine motifs are separated by another amino acid residue. In the second subfamily, designated C-C or β, the first cysteines are adjacent. C subfamily members, also designated γ chemokines, lack the first and third cysteine residues of the conserved motif. In the C-X3-C, or δ subfamily, members have three amino acids between the two cysteines. The C-X-C chemokine subfamily includes IL-8, GROα/β/γ (and the murine homologs KC, MIP-2α and MIP-2β), platelet basic protein, ENA-78, GCP-2, PF4, IP-10 (and its murine homolog, CRG) and MIG.