PRODUCT CODE: ET1706-32

Recombinant Involucrin Monoclonal Antibody (ET1706-32)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

-
+
Western blot analysis of Involucrin on human skin tissue lysate using Involucrin antibody at 1/500 dilution.
  • Western blot analysis of Involucrin on human skin tissue lysate using Involucrin antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Involucrin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A431 cells with Involucrin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Involucrin on human skin tissue lysate using Involucrin antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Involucrin Monoclonal Antibody (ET1706-32)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human skin tissue lysate, human lung cancer tissue, A431 cell.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU73-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

120 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Involucrin

SYNONYMS

INVO_HUMAN antibody; Involucrin antibody; IVL antibody

SEQUENCE SIMILARITIES

Belongs to the involucrin family.

TISSUE SPECIFICITY

Keratinocytes of epidermis and other stratified squamous epithelia.

POST-TRANSLATIONAL MODIFICATION

Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Involucrin is a precursor protein of the keratinocyte cornified envelope, which is formed beneath the inner surface of the cell membrane during terminal differentiation. During keratinocyte terminal differentiation, glutamine residues of involucrin become covalently cross-linked to other envelope precursors via covalent epsilon-(gamma-glutamyl) lysine bonds. Moreover, its large size allows involucrin to cross-link molecules that are separated by substantial distances in the cornified envelope. These properties allow a single involucrin molecule to form multiple cross-links, in multiple spatial planes, with other envelope precursors.