PRODUCT CODE: ET1610-76

Recombinant Integrin linked ILK Monoclonal Antibody (ET1610-76)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Integrin linked ILK on different lysates using anti-Integrin linked ILK antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Lane 1: Hela <br />
  Lane 2: 293T
  • Western blot analysis of Integrin linked ILK on different lysates using anti-Integrin linked ILK antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Lane 1: Hela <br />
  Lane 2: 293T
  • ICC staining Integrin linked ILK in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Integrin linked ILK in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Integrin linked ILK antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with Integrin linked ILK antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Integrin linked ILK on different lysates using anti-Integrin linked ILK antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: 293T

Applications

  • WB

  • ICC

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Integrin linked ILK Monoclonal Antibody (ET1610-76)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NIH/3T3, SH-SY-5Y, Hela, rat brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC68-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

51 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Integrin linked ILK

SYNONYMS

59 kDa serine/threonine protein kinase antibody; 59 kDa serine/threonine-protein kinase antibody; DKFZp686F1765 antibody; Epididymis secretory protein Li 28 antibody; HEL S 28 antibody; ILK 1 antibody; ILK 2 antibody; ILK antibody; ILK-1 antibody; ILK-2 antibody; ILK_HUMAN antibody; ILK1 antibody; ILK2 antibody; Integrin linked kinase 2 antibody; Integrin linked Kinase antibody; Integrin linked protein kinase antibody; Integrin-linked protein kinase antibody; p59 antibody; p59ILK antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

TISSUE SPECIFICITY

Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylated on serine residues.

SUBCELLULAR LOCATION

Cell junction, Cell membrane, Cytoplasm.

FUNCTION

Integrins are heterodimers composed of non-covalently associated transmembrane a and b subunits. The 16 a and 8 b subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind to ligands that are components of the extracellular matrix. Certain integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. ILK (integrin-linked kinase) was identified as a serine/threonine kinase that phosphorylates b1 and b3 integrins. ILK expression has been shown to be reduced in response to Fibronectin, a known integrin ligand. Overexpression of ILK was shown to upregulate the Fibronectin matrix assembly in epithelial cells, indicating a potential role for ILK in cell growth, cell survival and tumorigenesis.