PRODUCT CODE: ET1703-52

Recombinant Integrin beta 4 Monoclonal Antibody (ET1703-52)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

using anti-Integrin beta 4 antibody at 1/500 dilution.<br />
Positive control: <br />
Line 1: A431 <br />
Line 2: A549
  • using anti-Integrin beta 4 antibody at 1/500 dilution.<br />
Positive control: <br />
Line 1: A431 <br />
Line 2: A549
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Integrin beta 4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Integrin beta 4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Integrin beta 4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Integrin beta 4 antibody. Counter stained with hematoxylin.
using anti-Integrin beta 4 antibody at 1/500 dilution.
Positive control:
Line 1: A431
Line 2: A549

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Integrin beta 4 Monoclonal Antibody (ET1703-52)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, A549, human breast cancer tissue, human tonsil tissue, human pancreas tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-06

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

210 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Integrin beta-4

GENE NAME

ITGB4

SYNONYMS

ITGB4

SEQUENCE SIMILARITIES

Belongs to the integrin beta chain family.

TISSUE SPECIFICITY

Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.

POST-TRANSLATIONAL MODIFICATION

Palmitoylated by DHHC3 at several cysteines of the membrane-proximal region, enhancing stability and cell surface expression. Palmitoylation also promotes secondary association with tertaspanins.

SUBCELLULAR LOCATION

Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Cell junction, hemidesmosome. Note=Colocalizes with DST at the leading edge of migrating keratinocytes.

FUNCTION

Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling.