PRODUCT CODE: ET1611-27

Recombinant Integrin alpha E Monoclonal Antibody (ET1611-27)

  • Recombinant

Applications

  • IHC-P

REACTIVITY

  • Human

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Integrin alpha E antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Integrin alpha E antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Integrin alpha E antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Integrin alpha E Monoclonal Antibody (ET1611-27)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN62-06

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

Isotype

IgG

APPLICATION DILUTION

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Integrin alpha E

SYNONYMS

A530055J10 antibody; alpha-E1 antibody; alpha-M290 antibody; aM290 antibody; Antigen CD103 antibody; CD 103 antibody; CD103 antibody; CD103 antigen antibody; HML 1 antigen antibody; HML-1 antigen antibody; HML1 antigen antibody; Human mucosal lymphocyte antigen 1 antibody; Human mucosal lymphocyte antigen 1, alpha subunit antibody; Huminae antibody; Integrin alpha E heavy chain antibody; Integrin alpha E light chain antibody; Integrin alpha E, epithelial-associated antibody; Integrin alpha E1 antibody; Integrin alpha IEL antibody; Integrin alpha M290 antibody; Integrin alpha-E heavy chain antibody; Integrin alpha-IEL antibody; Integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide) antibody; Integrin, alpha E antibody; ITAE_HUMAN antibody; Itgae antibody; MGC141996 antibody; MGC183601 antibody; Mucosal lymphocyte 1 antigen antibody; Mucosal lymphocyte antigen 1 antibody

SEQUENCE SIMILARITIES

Belongs to the integrin alpha chain family.

TISSUE SPECIFICITY

Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

Integrins are heterodimers composed of noncovalently associated transmembrane a and b subunits. The 16 a and 8 b subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, Collagen and Vitronectin. Certain integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.